蕨菜提取物抗肥胖作用及其机制

Anti-obesity Effect of Pteridium aquilinum Extract and Its Mechanism

研究蕨菜对3T3-L1前脂肪细胞分化和高脂饮食诱导C57BL/6J小鼠肥胖的抑制作用,初步探讨其作用机制。培养3T3-L1前脂肪细胞并诱导其分化,油红O染色比色分析蕨菜乙醇提取物(PAE)对其分化程度的影响,Western blot法检测其对脂肪细胞分化转录调控因子(PPARγ、CEBPα、SREBP-1c)与脂质合成相关基因(FAS、ACC)的表达水平。以高脂饮食6周建立C57BL/6J肥胖小鼠模型,将诱导成功的肥胖小鼠分为高脂对照组(HFD)、1%蕨菜乙醇提取物组(HFD+PAE),另设立正常饲料喂养的C57BL/6J小鼠为正常组(ND)。继续喂养8周后测定各组小鼠体质量,评价其脂代谢状况(甘油三酯、胆固醇)及糖代谢情况(血糖、葡萄糖耐量)。结果显示,PAE能显著抑制3T3-L1前脂肪细胞分化,减少细胞中脂质的沉积。PAE显著影响脂肪细胞分化过程中细胞分化转录调控因子PPARγ、CEBPα、SREBP-1c的蛋白表达(P<0.05),同时降低脂质合成相关基因FAS、ACC的表达(P<0.05)。经过高脂饮食诱导C57BL/6J肥胖小鼠体质量增加,体脂沉积显著,血糖、血脂水平明显高于ND组(P<0.05)。PAE干预后,小鼠体质量、甘油三酯、血糖水平均较HFD明显降低(P<0.05),葡萄糖耐量也得到显著改善(P<0.05)。蕨菜乙醇提取物抑制了3T3-L1脂肪细胞分化,改善了肥胖小鼠体质量,消除了高血脂和高血糖异常,修复了葡萄糖耐量,这一作用可能与其调控脂肪细胞分化转录调控因子和脂质合成相关基因等密切相关。

Anti-obesity effect of Pteridium aquilinum and its mechanism was investigated by measuring its inhibitory effect against 3T3-L1 preadipocyte differentiation and high fat diet induced C57BL/6J obese mice.The differentiation of 3T3-L1 cells was induced and Oil Red O staining was used to analyze the effect of Pteridium aquilinum ethanol extract(PAE)on the differentiation of 3T3-L1 preadipocytes.To elucidate the mechanism of PAE,we performed Western blot for the expression of genes related with adipogenesis(PPARγ,CEBPα,SREBP-1c)and lipogenesis(FAS,ACC).C57BL/6J mice were induced by high fat diet for 6 weeks.Obese C57BL/6J mice were then randomly divided into high-fat diet group(HFD)and the 1%PAE group(HFD+PAE).In addition,C57BL/6J mice fed with normal diet were selected as the normal diet group(ND).After 8 weeks,the body weight,glucose metabolism indications(blood glucose,glucose tolerance)and serum lipid levels(TG,TC)in all mice were evaluated.The results showed that PAE suppressed 3T3-L1 adipocyte differentiation and reduced the accumulation of fat in adipocytes.Treatment of PAE markedly suppressed the protein expression of PPARγ,CEBPαand SREBP-1c,as well as FAS and ACC(P<0.05),which are the key transcription factors and metabolic enzymes in adipogenesis and lipogenesis.After 8-weeks feeding,the indicator in HFD group were high than ND group,including body weight,body fat deposition,serum glucose and serum lipid(P<0.05).Treatment with PAE significantly decreased the body weight,blood glucose and the levels of serum TC,TG.PAE also effectively improved impaired glucose tolerance(P<0.05).In conclusion,Pteridium aquilinum suppressed 3T3-L1 adipocyte differentiation,inhibited obesity,hyperglycemia,hyperlipemia and improved impaired glucose tolerance in C57BL/6J obese mice.These effects might be closed related to its inhibition of genes expression regulating adipogenesis and lipogenesis.