4种不同拭子释放人细胞DNA效果的研究

Effect of Four Different Swabs on DNA Release from the Adhered-to-swab Human Cells

目的比较4种不同拭子上附着的细胞释放DNA的效果。方法在4N6FLOQSwabsTM植绒拭子、生物检材采集与保存套管、生物物证提取专用棉签拭子以及普通医用棉签拭子上分别滴加5μL全血,制备成原始有核细胞含量基本一致的拭子各40份,将上述拭子根据保存时间不同分为A组(室温1d,4×20根)与B组(室温30d,4×20根),采用改良硅珠法提取DNA后应用定量PCR仪检测所提取的模板DNA量,最后用SPSS统计学软件对数据进行分析。结果4N6FLOQSwabs^TM植绒拭子所释放的模板DNA量最高,可提高后续DNA检验的成功率;放置30d后虽然模板DNA量有所下降,但所释放的DNA量仍优于其他3种拭子。结论在法医实际案件检验中,尤其对载体上极微量人体细胞的收集应优先选择植绒拭子进行收集及保存,并尽快检验。

Objective To compare the effect of four different swabs on releasing DNA from the adhered-to-swab human cells.Methods 5μL evenly-dispersed whole blood was respectively dropped onto 4N6FLOQSwabsTM flocking swabs(40 pieces),biological sample collecting-and-preserving casings(40 ones),forensic cotton swabs(40 ones)and common medical cotton swabs(40 ones).All the swabs were divided into group A(placing at room temperature for 1 day,4×20 pieces)and group B(placing at room temperature for 30 days,4×20 pieces).The template DNA was extracted with the modifi ed silica bead method,and followed to undergo the quantitative PCR to test the amount of extracted DNA,with the final data being analyzed by SPSS statistical software.Results The maximal amount of template DNA was released from the 4N6FLOQSwabs^TM flocking swabs,having the success rate of subsequent DNA test improved.Even after 30 days,the amount of DNA released by the 4N6FLOQSwabs^TM flocking swab,albeit declining,remained higher than the other three handlingdifferent swabs.Conclusion For the actual forensic cases,the priority should be given to the 4N6FLOQSwabs^TM flocking swab for sample collection and preservation,especially the tiny quantity of human cells.Additionally,the collected samples ought to be tested as early as possible.