均匀设计法优选湖北贝母生物碱提取工艺

Optimum Extraction Process of Alkaloids from Hubei Fritillaria by Uniform Design

目的:筛选湖北贝母生物碱提取最佳工艺条件。方法:采用均匀设计法,以乙醇浓度、液料比、提取时间、提取次数为考察因素,以浸膏得率和生物碱收率为考察指标,对湖北贝母生物碱乙醇提取工艺进行优化。结果:湖北贝母生物碱最佳提取工艺为80%乙醇、液料比10倍、提取时间1h、提取次数3次,生物碱收率为0.5164%。

In order to screen out the optimum conditions for the extraction of alkaloids from Fritillaria chinensis Maxim.,the uniform design method was used.The ethanol concentration,liquid-to-liquid ratio,extraction time and extraction times were taken as the investigation factors,and the extraction yield and alkaloid yield were taken as indicators.To optimize the ethanol extraction process of the Fritillaria alkaloids in Hubei.The results showed that the best extraction process of alkaloids from Fritillaria chinensis was 80%ethanol,the ratio of liquid to liquid was 10 times,the extraction time was 1 hour,the extraction times were 3 times,and the alkaloid yield was 0.5164%.