植物乳杆菌ER135对利血平诱导抑郁小鼠的改善作用

Effect of Lactobacillus plantarum ER135 on the Improvement of Depression Induced by Reserpine in Mice

为了研究植物乳杆菌(Lactobacillus plantarum)ER135对利血平诱导抑郁小鼠的改善作用,选择6周龄雄性ICR小鼠,连续21 d灌胃植物乳杆菌ER135菌悬液(2.0×10^10 cfu/mL),第22 d小鼠腹腔注射利血平(4 mg/kg)构建抑郁模型,通过小鼠行为学观察,血清和脑组织生化指标检测及海马组织蛋白表达变化,评价植物乳杆菌ER135对抑郁模型小鼠的改善作用。结果表明,饲喂植物乳杆菌ER135显著改善小鼠体温降低、眼睑下垂和运动能力下降等症状。与模型组相比,脑组织中5-羟色胺、多巴胺、去甲肾上腺素等神经递质的浓度分别提高了2.06 ng/L、1.42 pg/L、10.12 ng/L。降低血清炎症因子水平,肿瘤坏死因子以及白介素1β分别降低了9.40 ng/L和10.63 ng/L,同时还降低了氧化应激水平,与模型组相比差异显著(p<0.05,p<0.01)。海马组织Western-blot结果显示植物乳杆菌ER135明显增加抑郁小鼠海马组织神经营养通路中cAMP、CREB、BDNF等关键蛋白的表达。综上所述植物乳杆菌(Lactobacillus plantarum)ER135具有缓解利血平诱导抑郁的作用。

The improvement effect of Lactobacillus plantarum ER135 on depression induced by reserpine in mice was investigated.Male ICR mice(n=40,6-week)were administered with L.plantarum ER135 strain suspension(2.0×10^10 cfu/mL)by gavage for continuous 21 days,then all mice were injected with reserpine(4 mg/kg)at 22th day to establish depression model.The improvement effect of L.plantarum ER135 on the mice was evaluated by observing the changes of behavioristics phenomena of mice,detecting serum and tissue biochemical indicator as well as relevant protein expression changes in hippocampal tissue.Compared with the control group,after administration of L.plantarum ER135,the symptoms of hypothermia,blepharoptosis and decreased motor ability of these mice were significantly improved.Compared with the model group,the concentration of 5-hydroxytryptamine,dopamine and norepinephrine in brain tissue increased by 2.06 ng/L,1.42 pg/L and 10.12 ng/L,respectively.The level of serum inflammatory factors decreased.Tumor necrosis factor and interleukin-1 beta decreased by 9.40 ng/l and 10.63 ng/L,respectively.It also reduced the level of oxidative stress(p<0.05,p<0.01).Western-blot results showed that L.plantarum ER135 significantly could incuce an increases of the expression of cAMP,CREB,BDNF and other key proteins in the hippocampal neurotrophic pathway of depressed mice.L.plantarum ER135 can relieve depression of mice induced by reserpine.