摘要
Soybean cyst nematode(SCN)is a highly destructive pathogen.The soybean host genome harbors at least two major genes for resistance(rhg1 and Rhg4),as well as a minor locus(SCN3-11).In the present study,a splicing site in GmSNAP11,the potential causal gene of SCN3-11,was identified by comparison of the GmSNAP11 cDNA sequences generated from resistant and susceptible soybean accessions.The sequence information was used to design a codominant CAPS marker,GmSNAP11-2565,which was used to genotype a panel of 209 soybean accessions varying with respect to SCN resistance.Analyses of the effect of the haplotypes formed by GmSNAP11-2565 and another large-effect(nonsynonymous)locus,GmSNAP11-2307,previously identified in GmSNAP11,revealed linkage disequilibrium(P<0.0001)between the two loci,suggesting that GmSNAP11-2565 could be used as a marker for GmSNAP11.GmSNAP11-2565 was accordingly used,along with established markers for GmSNAP18(rhg1)and GmSHMT(Rhg4),to characterize the panel accessions.The mean SCN female index of accessions carrying only the GmSNAP11 allele associated with resistance(20.3%)was higher than that associated with accessions carrying alleles for resistance at both GmSNAP11 and GmSNAP18(12.4%),while the index for accessions carrying alleles for resistance at all of GmSNAP11,GmSNAP18,and GmSHMT was very low(1.9%).Selection on all three markers was effective for maintaining a high level of resistance to SCN race 3.
Soybean cyst nematode(SCN) is a highly destructive pathogen. The soybean host genome harbors at least two major genes for resistance(rhg1 and Rhg4), as well as a minor locus(SCN3-11). In the present study, a splicing site in Gm SNAP11, the potential causal gene of SCN3-11, was identified by comparison of the Gm SNAP11 c DNA sequences generated from resistant and susceptible soybean accessions. The sequence information was used to design a codominant CAPS marker, Gm SNAP11-2565, which was used to genotype a panel of 209 soybean accessions varying with respect to SCN resistance. Analyses of the effect of the haplotypes formed by Gm SNAP11-2565 and another large-effect(nonsynonymous)locus, Gm SNAP11-2307, previously identified in Gm SNAP11, revealed linkage disequilibrium(P < 0.0001) between the two loci, suggesting that Gm SNAP11-2565 could be used as a marker for Gm SNAP11. Gm SNAP11-2565 was accordingly used, along with established markers for Gm SNAP18(rhg1) and Gm SHMT(Rhg4), to characterize the panel accessions. The mean SCN female index of accessions carrying only the Gm SNAP11 allele associated with resistance(20.3%) was higher than that associated with accessions carrying alleles for resistance at both Gm SNAP11 and Gm SNAP18(12.4%), while the index for accessions carrying alleles for resistance at all of Gm SNAP11, Gm SNAP18, and Gm SHMT was very low(1.9%). Selection on all three markers was effective for maintaining a high level of resistance to SCN race 3.
基金
National Key R&D Program for Crop Breeding (2016YFD0100602, 2016YFD0100201)
the Agricultural Science and Technology Innovation Program (ASTIP) of the Chinese Academy of Agricultural Sciences
National Science and Technology Platform
