玉米花序发育相关基因ZmSPI1的序列变异分析

Sequence Variation Analysis of Maize Flower Inflorescence Related Gene ZmSPI1

玉米ZmSPI1基因编码的黄素单加氧酶,是单子叶植物特有的,该基因通过参与生长素的合成过程,从而影响花序的发育。本研究在103份玉米自交系中,对该基因进行重测序,获得了全长序列1 849 bp。核苷酸多态性分析发现,该基因共检测到43个变异位点,变异类型均为SNP。该基因编码区具有6个SNP位点,划分成9种编码区单倍型,均为同义突变。中性检测结果表明该基因在供试群体中没有受到明显的人工选择信号。

The flavin monooxygenase encoded by the maize ZmSPI1 gene is unique to monocotyledons,that affects the development of inflorescences by participating in the synthesis of auxin.ZmSPI1 gene was captured in 103 elite maize inbred lines,and the total sequence was 1 849 bp.The nucleotide polymorphism analysis revealed that 43 mutation sites were detected in the locus,and the mutation types were SNPs.The coding region of the gene had six SNP sites and was divided into nine coding region haplotypes,all of region haplotypes were synonymous mutations.The neutral test results showed that the gene was not subjected to artificial selection signals significantly in the test population.