酶解预处理对水酶法提取牡丹籽油提油率的影响

Effect of Enzymolysis Pretreatment on Peony Seeds Oil Extraction Rate by Aqueous Enzymatic Method

为提高水酶法提取牡丹籽油的出油率,研究了果胶酶和纤维素酶复合酶预处理对提油量的影响,结果表明:果胶酶与纤维素酶比例为2∶1时,提油量最高;通过单因素及响应曲面试验设计优化得到复合酶酶解预处理牡丹籽提油的最佳工艺条件为:pH 4.3、复合酶添加量2.5%、酶解温度47℃、酶解时间2.3h。该工艺参数下进行前处理,再用碱性蛋白酶酶解,每10g牡丹籽的提油量为1.345 8g,换算成提油率约为85%。

The effect of pretreatment with complex enzymes of pectinase and cellulase on peony seeds oil extraction rate was studied.The results showed that the peony seeds oil extraction rate was the highest when the ratio of the amount of pectinase to cellulase was 2∶1.The optimal conditions for pretreatment of peony seeds for oil extra ction by single-factor tests and response surface method were pH 4.3,enzyme dosage 2.5%,enzymolysis temperature 47℃,and enzymolysis time 2.3 h.Under the above pretreatment conditions combined with alkaline protease enzymolysis,the peony seeds oil extraction rate was up to 1.345 8g per 10g,and the oil extraction rate was about 85%.