人宫颈癌中CPE△N的表达及对肿瘤细胞增殖迁移功能的影响

Expression of CPE△N in human cervical cancer and its influence on proliferation,migration of tumor cells

目的:探讨N端截短的羧肽酶E(N-terminal truncated carboxypeptidase E,CPE△N)在人宫颈癌中的表达情况及其对肿瘤细胞增殖迁移功能的影响。方法:采用qRT-PCR方法检测35例宫颈癌患者肿瘤组织中CPE△N的表达水平,同时检测CPEΔN高表达组肿瘤细胞增殖和迁移功能并进行相关分析。结果:宫颈癌患者肿瘤组织CPEΔN mRNA/18sRNA比值平均为2.01±0.65,显著高于对照组,其高表达情况与细胞迁移功能具有显著相关(P<0.05)。结论:宫颈癌组织中CPE△N过表达并促进肿瘤细胞的迁移,由此推断CPE△N极有可能作为宫颈癌患者病情发展和预后评价的生物标志物。

Objective We detected the expression of CPE△N in human cervical cancer and analysed the influence on proliferation,migration of tumor cells,evaluated the probability as a potential prognostic biomarker of cancer.Method We analyzed the expression of CPE△N in human cervical cancer by qRT-PCR in 35 patients;MTT assay,healing assay were used to detect the proliferation and migration of tumor cells which high-expressed CPE△N.Results The average ratio of CPE△N mRNA to 18sRNA in cervical cancer was 2.01±0.65.The results showed that CPE△N was overexpression in human cervical cancer,and that high expression was correlated with migration of tumor cells(P<0.05).Conclusion Basing on our results,high CPE△N was indentified as an independent prognotic factor,this conclusion can be arrivaled that CPE△N might be a potential profnostic marker for tumor patients.