肝细胞癌中lncRNA-CAI2的表达及临床意义

Expression and clinical significance of lncRNA-CAI2 in hepatocellular carcinoma

目的探讨lncRNA-CAI2(CDKN2A/ARF/Intron 2 non-coding RNA)在原发性肝细胞癌中的表达及临床意义。方法收集92例手术切除的肝细胞癌及46例癌旁肝组织标本,利用实时荧光定量PCR法检测CAI2 mRNA表达水平,分析其与临床病理特征及生存期的相关性。结果CAI2在肝细胞癌组织中的表达水平明显高于癌旁肝组织,差异有统计学意义;CAI2高表达率与组织学分级、临床分期、是否有门脉癌栓明显相关,高表达患者的无瘤生存期和总生存期均明显低于低表达者;CAI2高表达与门脉侵犯和临床分期联合分析对肝细胞癌的无瘤生存期和5年总生存期均有明显影响;CAI2高表达是肝细胞癌患者预后不良的独立风险因子。结论lncRNA-CAI2可作为评估肝细胞癌预后不良的潜在标志物。

Purpose To study the expression and clinical significance of lncRNA-CAI2(CDKN2A/ARF/Intron 2 non-coding RNA)in hepatocellular carcinoma(HCC).Methods 92 cases of the HCC and 46 cases of adjacent non-tumor tissue undergoing surgical operation were selected.CAI2 expression was detected by RT-PCR method.Moreover,the correlation of the CAI2 with clinicopathologic parameters and survival of patients was analysed by statistical methods.Results The expression level of CAI2 in HCC was obviously higher than that of adjacent non-tumor tissue.The high expression level of CAI2 in HCCs was obviously related to histologic grade,clinical stage and portal vein tumor thrombus.Additionally,disease-free survival and 5-year overall survival rates of HCC with CAI2 high-expression were significantly lower than those with CAI2 low expression.The combined analysis of high-expression of CAI2,portal vein invasion and clinical stage had significant effect on the disease-free survival rate and 5-year overall survival rate of HCC.CAI2 high-expression was an independent risk factor of poor prognosis for HCC patients.Conclusion LncRNA-CAI2 may be used as a potential marker for evaluating the poor prognosi s of HCC.