人黑色素瘤抗原A3在宫颈癌组织和细胞中的表达研究

Expression of the melanoma-associated antigen-A3(MAGE-A3) in cervical cancer tissues and cervical cancer cells

目的:深入研究人黑色素瘤相关抗原A3(MAGE-A3)在宫颈癌(CC)组织和CC细胞系中的表达情况,探讨其在CC治疗中的意义。方法:应用免疫组织化学SP法检测MAGE-A3在40例CC组织和20例正常宫颈组织中的表达。采用实时荧光定量PCR(RT-qPCR)法检测MAGE-A3 mRNA在3种不同CC细胞株及正常宫颈细胞株中的表达情况。应用Western blot检测MAGE-A3蛋白在3种不同CC细胞株(HeLa、SiHa和C-33A)及正常宫颈细胞株(End1/E6E7)中的表达水平。结果:MAGE-A3在正常宫颈组织中不表达,在CC组织中有表达,阳性表达率分别为0%和70%。MAGE-A3 mRNA和蛋白在3种CC细胞株中的表达明显高于正常宫颈细胞株(P<0.05)。结论:MAGE-A3在CC组织和CC细胞中高表达,提示在CC的发生发展中MAGE-A3可能具有重要作用。

Objective To further elucidate the expression of melanoma-associated antigen-A3(MAGE-A3)in cervical cancer tissues and cervical cancer cell lines,and to explore its significance in the treatment of cervical cancer.Methods Immunohistochemical SP method was used to detect the expression of MAGE-A3 in 40 samples of cervical cancer tissue and 20 samples of normal cervical tissue.The expression of MAGE-A3 mRNA in three different cervical cancer cell lines and normal cervical cell line was detected by real-time fluorescence quantitative PCR(RT-qPCR).The expression levels of MAGE-A3 protein in three different cervical cancer cell lines-HeLa,SiHa and C-33A and normal cervical cell line were detected by Western blot.Results MAGE-A3 was not expressed in normal cervical tissues and was expressed in cervical cancer tissues.The positive expression rates were 0%and 70%,respectively.The expressions of MAGE-A3 mRNA and protein in three cervical cancer cell lines were significantly higher than those in normal cervical cancer cell lines.Conclusion MAGE-A3 is highly expressed in cervical cancer tissues and cervical cancer cells.It suggests that MAGE-A3 plays an important role in the development of cervical cancer.