氟化钠对掺锶透钙磷石骨水泥成骨活性的影响

Study on the effect of sodium fluoride on the osteogenic potential of doped-strontium brushite bone cement

探究掺入不同质量氟化钠至锶透钙磷石骨水泥对理化性能及成骨活性的影响。以二水合磷酸氢钙与碳酸钙为原料制备β-磷酸三钙,以氯化锶为原料制备掺锶透钙磷石骨水泥,考察将氟化钠按不同质量比(0.5wt%、1.0wt%、1.5wt%)制备的掺氟锶透钙磷石骨水泥微观形貌、元素含量、物相形式及抗压强度,MTT法检测细胞增殖,并对其碱性磷酸酶(ALP)和骨保护素OPG mRNA表达进行检测。掺氟锶透钙磷石骨水泥抗压强度低于单一掺锶透钙磷石骨水泥(P>0.05),当氟化钠质量比为1.5wt%时促进MC3T3-E1的增殖及ALP效果最好,1.0wt%时显著促进OPG蛋白表达。

To incorporate sodium fluoride with different mass ratios into doped-strontium calcium phosphate stone cement to test its physical and chemical properties and its effect on osteogenic activity.β-tricalcium phosphate was synthesized from calcium hydrogen phosphate dihydrate and calcium carbonate,and strontium-doped calcium phosphate stone cement was prepared with SrCl 2,The morphology,element content,phase form and compressive strength of fluorine-dopedyttrium-calcium phosphate cement prepared by sodium fluoride at different mass ratios(0.5wt%,1.0wt%,1.5wt%)were investigated.MTT method cell proliferation was measured and its alkaline phosphatase(ALP)and osteoprotegerin OPG mRNA expression was examined.The compressive strength of doped F/Sr brushite bone cement was lower than that of single doped Sr brushite bone cement(P>0.05),when sodium fluoride was 1.5wt%,the proliferation and ALP effect of MC3T3-E1 were promoted,the expression of OPG protein was significantly promoted when it was 1.0wt%.