黄芪甲苷对CCL4所致肝损伤大鼠的保护作用

Protective effects of astragaloside in rats with CCL4-induced liver injuries

目的探讨黄芪甲苷对CCL4所致肝损伤大鼠的保护作用。方法将50只SD大鼠随机分为对照组、模型组、小剂量、中等剂量和大剂量黄芪甲苷处理组,在造模成功后除对照组和模型组外,给予药物灌胃。取大鼠血清和肝组织,检测肝组织匀浆超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、丙二醛(MDA)含量及血生化和羟脯氨酸(Hyp)含量;采用TUNEL法检测肝细胞凋亡。结果与模型组比,小、中、大剂量黄芪甲苷处理组动物肝组织匀浆SOD、GSH和MDA水平得到显著改善(P<0.05),血清ALT、AST、TBIL和Hyp含量也显著降低(P<0.05),而ALB含量显著升高(P<0.05);小剂量黄芪甲苷组肝组织凋亡小体数为(55.36±2.15)个,中剂量黄芪甲苷组为(44.58±3.06)个,大剂量黄芪甲苷组为(33.24±3.18)个,均显著低于模型组的(66.54±2.56)个(P<0.05);肝组织病理学检查结果显示,与模型组比,小、中、大剂量黄芪甲苷处理组大鼠肝组织损伤表现显著改善。结论黄芪甲苷对CCL4所致肝损伤具有很好的保护作用。

Objective To explore the protective effects of astragaloside in rats with CCL4-induced liver injuries.Methods 50 SD rats were randomly divided into control,model,low-dose(40 mg·kg^-1),middle-dose(80 mg·kg^-1)and large-dose(160 mg·kg^-1)of astragaloside-intervened groups.After successful modeling,the astragaloside was administered intragastricly except rats in control and model group.Serum parameters,hydroxyprolin(Hyp)and liver tissue homogenate superoxide dismutase(SOD),glutathione(GSH)and malondialdehyde(MDA)were detected.The hepatocyte apoptosis was detected by TUNEL.Results Compared with in model group,liver tissue SOD,GSH and MDA and blood ALT,AST,bilirubin levels in low-dose,middledose and large-dose of astragaloside groups were significantly improved(P<0.05),serum Hyp levels were significantly decreased(P<0.05),and albumin levels were significantly increased(P<0.05);the apoptotic bodies in liver tissues were(55.36±2.15),(44.58±3.06)and(33.24±3.18),respectively in low-dose,in middle-dose and in large-dose of astragaloside-intervened groups,significantly lower than(66.54±2.56)in the control(P<0.05);liver histopathologic results showed that the cellular cord disorder,cellular hydropic degeneration and inflammatory cell infiltration in the liver tissues were significantly improved in low-dose,middle-dose and large-dose of astragaloside groups.Conclusions Astragaloside has a good protective effect in rats with CCL4-induced liver injuries.