利用iTRAQ蛋白质组技术筛选福寿螺抗药相关的候选蛋白

Identification of candidate proteins related to molluscicide resistance in apple snail,Pomacea canaliculata based on iTRAQ analysis

采用同位素标记相对和绝对定量(isobaric tags for relative and absolute quantitation,iTRAQ)技术对杀螺剂胁迫下福寿螺肝脏蛋白质组进行研究,以期从蛋白质组学水平了解福寿螺对杀螺剂四聚乙醛的胁迫应答。结果鉴定到108个具有定量信息的差异蛋白(P<0.05),其中显著上调的蛋白有55个,下调蛋白53个。理化性质分析和GO(gene ontology)注释分析发现,这些蛋白分别具有抗氧化、催化结合、抗菌等功能,并影响福寿螺的细胞结构组成、肌肉收缩、代谢调节和免疫调节,引起神经毒性。研究测定出57个具有不同活性的细胞色素450酶系蛋白(CYP450),推测CYP450活性是福寿螺对四聚乙醛产生抗药性的重要原因。

The stress response of Pomacea canaliculata to molluscicide metaldehyde at proteomic level was analyzed in this study,which lays a foundation for future study of the molecular mechanism of resistance to drugs in snail.Proteome of the P.canaliculata liver was studied using the isobaric tags for relative and absolute quantitation(iTRAQ)technology.The results showed that 108 differentially expressed proteins(P<0.05)with quantitative information were identified,of which 55 were significantly up-regulated and 53 were down-regulated.The physical and chemical properties analysis and GO(gene ontology)annotation analysis showed that these proteins could be functionally categorized into antioxidant,catalytic and antibacterial functions,affecting the cellular structure composition,muscle contraction,metabolic regulation and immunoregulation and causing neurotoxicity of snail.All the 57 Cytochrome P450 were found to show different expression activity,suggesting that CYP450 may contribute to metaldehyde resistance of P.canaliculata.