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湖北地方品种江汉鸡种鸡禽白血病净化初报

Preliminary report on purification of avian leukosis in Hubei local Jianghan chicken
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摘要 因地方鸡种禽白血病病毒(Avian leukosis virus,ALV)感染的严峻形势,对江汉鸡4个种群4个世代开展了禽白血病的检测与净化研究。结果表明,通过对不同发育阶段、不同性别的群体,采集不同的样品连续进行了4年的检测和净化,江汉鸡种群纯繁前(40~45周龄)公鸡精液ALV p27抗原检测平均阳性率从2015年的30.54%降至2018年的0.23%,母鸡蛋清样品ALV p27抗原检测平均阳性率从2015年的26.98%降至2018年的0.05%。在育雏结束、开产和纯繁前3个时间节点进行ALV p27抗原的检测,淘汰阳性个体,禽白血病净化效果明显,可为其他地方鸡种禽白血病的净化工作提供参考。 Based on the severe situation of avian leukosis virus infection in local chicken breeds,in this study,four generations of four Jianghan chicken populations were tested and purified for avian leukemia.The results showed that different samples were collected from different developmental stages and different sex groups for four consecutive years for detection and purification,the average positive rate of ALV p27 antigen in cock semen before pure breeding(40-45 weeks old)decreased from 30.54%in 2015 to 0.23%in 2018.The average positive rate of ALV p27 antigen in hen egg white samples decreased from 26.98%in 2015 to 0.05%in 2018.It can be seen that the detection of ALV p27 antigen at three time points before the end of brooding,the start of production and pure breeding can eliminate positive individuals,and the purification effect of avian leukemia is obvious,which can provide reference for the purification of avian leukemia in other places.
作者 申杰 吴艳 皮劲松 潘爱銮 梁振华 孙静 张昊 蒲跃进 SHEN Jie;WU Yan;PI Jin-song;PAN Ai-luan;LIANG Zhen-hua;SUN Jing;ZHANG Hao;PU Yue-jin(Institute of Animal Husbandry and Vetervinary,Hubei Academy of Agricultural Science/Hubei Innovation Center of Agricultural Science and Technology/Hubei Key Lab of Animal Embryo Technology and Molecular Breeding,Wuhan 430064,China)
出处 《湖北农业科学》 2019年第20期125-127,共3页 Hubei Agricultural Sciences
基金 国家肉鸡产业技术体系建设专项资金项目(CARS-41-Z14) 动物胚胎与分子育种湖北省重点实验室开放课题(2018ZD100) 湖北省农业科技创新中心项目(2016-620-000-023)
关键词 江汉鸡 禽白血病 P27抗原 净化 Jianghan chicken avian leukosis p27 antigen purification
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  • 1龚新勇,陈红,李永明,温贵兰.蛋鸡J亚群禽白血病与马立克氏病混合感染的诊断[J].中国家禽,2007,29(15):50-51. 被引量:12
  • 2秦爱建.J-亚群鸡白血病病毒囊膜蛋白gp85基因及其产物的生物学和生物化学特性的鉴定:博士学位论文[M].扬州大学,1999..
  • 3秦爱建.禽白血病病毒J亚群囊膜糖蛋白基因的生物学和生物化学特征(博士学位论文)[M].扬州大学,2000..
  • 4张志.我国J亚群禽白血病病毒的流行病学调查和囊膜蛋白env基因的克隆和序列分析:硕士学位论文[M].山东农业大学,2001..
  • 5SailYM.禽病学[M].苏敬良,高福,索勋,主译.11版.北京:中国农业出版社,2005:518-578.
  • 6Smith E J, Fadly A, Okazaki W. An enzyme-linked immu- nosorbent assay for detection of avian leucosis- sarcoma viruses [J].Avian Disease, 1979,23(3) : 698-707.
  • 7Martin Himly, Douglas N Foster. The DF-1 Chicken Fibro- blast Cell Line: Transformation Induced by Diverse Oncogenes and Cell Death Resulting from Infection by Avian Leukosis Vi- ruses[J]. Virology,1998, 248:295-304.
  • 8杜岩 崔治中 等.从市场商品肉鸡检出J亚群禽白血病病毒[J].中国家禽学报,1999,3(1).
  • 9秦爱建,博士学位论文,1999年
  • 10Bai J,J Gen Virol,1995年,76卷,181页

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