NUAK1在鼻咽癌组织中的表达及其对鼻咽癌CNE2、HK1细胞迁移和侵袭能力的影响

Expression of NUAK1 in nasopharyngeal carcinoma and its effect on cell migration and invasion in human nasopharyngeal carcinoma CNE2 and HK1 cells

目的检测NUAK1在鼻咽癌及鼻咽黏膜慢性炎组织中的表达,进一步探讨沉默NUAK1对鼻咽癌CNE2、HK1细胞迁移和侵袭能力的影响。方法采用实时荧光定量PCR技术检测16例鼻咽癌和16例鼻咽黏膜慢性炎组织中NUAK1 mRNA的表达,免疫组织化学方法检测88例鼻咽癌及30例鼻咽黏膜慢性炎组织标本中NUAK1蛋白的表达,并分析NUAK1蛋白的表达与临床病理参数的相关性。应用siRNA技术转染CNE2、HK1细胞,随后进行Transwell迁移实验和侵袭实验,观察沉默NUAK1组相对空载组鼻咽癌细胞体外迁移和侵袭能力的改变。结果NUAK1 mRNA和蛋白在鼻咽癌中的表达均高于鼻咽黏膜慢性炎组织(P<0.05),且鼻咽癌中NUAK1蛋白的表达与淋巴结转移相关(P<0.05)。转染siRNA后,Transwell迁移实验和侵袭实验结果显示,实验组细胞的体外迁移和侵袭能力明显低于空载组(P<0.05)。结论NUAK1在鼻咽癌组织中高表达,在鼻咽癌CNE2、HK1细胞沉默NUAK1表达可抑制其迁移和侵袭,提示NUAK1的表达可能与鼻咽癌的侵袭转移有关。

Objective To detected the expression of NUAK1 in nasopharyngeal carcinoma(NPC)tissue and chronic inflammation of nasopharyngeal mucosa tissue,and further investigate the effect of silencing NUAK1 gene on cell migration and invasion in human NPC CNE2 and HK1 cells.Methods The expression levels of NUAK1 mRNA were detected by qRT PCR in 16 cases of nasopharyngeal carcinoma tissue and 16 cases of chronic inflammation of nasopharyngeal mucosa tissue.The expression levels of NUAK1 protein were detected by immuno histochemistry in 88 cases of nasopharyngeal carcinoma tissues and 30 cases of chronic inflammation of nasopharyn geal mucosa tissues.The association between the expression of NUAK1 protein and clinicopathologic features was evaluated.The NUAK1 siRNA and control siRNA were transfected into CNE2 and HK1 cells.The effect of silenc ing NUAK1 gene on cell migration and invasion in vitro wasanalyzed by transwell migration and invasion assay.Results The expression of NUAK1 mRNA and protein in NPC was significantly higher than those in chronic inflammation of nasopharyngeal mucosa tissue(P<0.05),and the expression of NUAK1 protein in NPC was correlated with lymph node metastasis.After transfecting siRNA into NPC cells,results showed that the ability of migration and invasion was lower in experimental group than those of the control group(P<0.05).Conclusions The expression levels of NUAK1 were significantly increased in NPC tissue.Silencing NUAK1 expression may suppress the migration and invasion of NPC CNE2 and HK1 cells,the expression of NUAK1 may be related to metastasis of NPC.