常压室温等离子体诱变筛选高产多糖猴头菌株的研究

Screening of high-yield polysaccharide Hericium erinareus by atmospheric and room temperature plasma mutagenesis

以猴头菌株0605为出发菌株,首次采用常压室温等离子体(ARTP)诱变技术选育高产胞内多糖的猴头菌株。结果表明:经筛选最终得到5株高产胞内多糖优良猴头菌株,其中菌株321的生产能力最佳。与出发菌株相比,优势菌株321的摇瓶发酵生物量提高了30.37%,胞内多糖含量提高了47.45%,且其菌丝多糖抗氧化活性和清除羟基自由基能力均优于出发菌株。拮抗实验和RAPD分析结果表明:优势菌株321的遗传物质发生了改变,证实其为突变菌株。高产多糖猴头菌株的获得为猴头菌相关产品的开发提供了新的资源,为利用常压室温等离子体(ARTP)技术诱变其他担子菌提供了参考。

In this study,atmospheric and room temperature plasma(ARTP)mutagenesis technology was first used to screen high-yield intracellular polysaccharide Hericium erinaceus,using strain 0605 as the original strain.The results showed that 5 strains of H.erinaceus with high-yield intracellular polysaccharide were obtained after screening,of which strain 321 had the best production capacity.Compared with the original strain,the shaking flask fermentation biomass of dominant strain 321 increased by 30.37%,and the intracellular polysaccharide content increased by 47.45%.The antioxidant activity and hydroxyl radical scavenging ability of mycelial polysaccharide of dominant strain 321 were better than those of the original strain.The results of antagonistic experiment and RAPD analysis showed that the genetic material of dominant strain 321 had changed,which confirmed that it was a mutant strain.The acquisition of high-yield polysaccharide strains of H.erinaceus provides new resources for the development of products related to H.erinaceus,and provides a reference for the mutagenesis of other basidiomycetes by ARTP.