云锦杜鹃转录组分析

Transcriptome sequencing and analysis of Rhododendron fortunei

云锦杜鹃Rhododendron fortunei具有很高的园艺观赏价值。由于缺乏相关的遗传信息,云锦杜鹃的多样性、分子辅助育种等工作进展较为缓慢。通过高通量测序技术(Illumina),对云锦杜鹃的转录组进行了测定和分析。通过测序拼接和去冗余共获得84 633条单基因簇(unigene)序列,平均长度为691.4 bp。通过与公共数据库比对成功注释到了35 526条单基因簇,其中与葡萄Vitis vinifera基因相似的序列最多。根据基因本体论数据库(GO)可将23 215个单基因簇归类于生物学过程、细胞组分及分子功能等三大类的55个功能组;真核直系同源基因数据库(KOG)将11 085条单基因簇分为26个功能分类,涉及一般功能预测的单基因簇最多,多达1 970条;以京都基因与基金组百科全书(KEGG)作为参考,依据代谢途径可将9 887个单基因簇定位到272个代谢通路。进一步分析基因注释结果,挖掘获得24个编码MADS-box基因的单基因簇分属于10个不同亚家族。此外,检测到了21 900个简单序列重复(SSR)位点,可用于SSR标记开发。

To enhance studies on genetic diversity and molecular assistance breeding of Rhododendron fortunei,one of the native Rhododendron species in China with a high horticultural and ornamental value,the transcriptome of R.fortunei was sequenced using Illumina technology.In addition,sequence assembly,public databases,Gene Ontology(GO)analysis,Eukaryotic Orthologous Groups(KOG),Kyoto Encyclopedia of Genes and Genomes(KEGG),and MADS-box transcription factor were used.Results of the sequence assembly obtained 84 633 unigenes with a mean length of 691.4 bp.Public databases were successfully searched annotating 35 526 unigenes,and GO analysis showed that 23 215 unigenes were potentially involved in 55 functional categories of biological processes,cellular components,and molecular functions.The KOG analysis indicated 11 085 unigenes classified into 26 functional groups;whereas,the KEGG analysis assigned 9 887 unigenes to 272 known pathways.Also,24 unigenes were identified to encode MADS-box transcription factor belonging to 10 different subfamilies.In addition,21 900 simple sequence repeat(SSR)loci were determined in the transcriptome of R.fortunei.In conclusion,this study could provide an important foundation for genetic diversity analysis,marker development,molecular assistant breeding,and functional gene isolation in R.fortunei,as well as future SSR marker development.[Ch,4 fig.5 tab.27 ref.]