角膜碱烧伤中PMNs与MMP-9的关系

Effect of PMNs and MMP-9 on corneal injury after alkali burn

目的:研究角膜碱烧伤后基质损伤修复病程中多形核中性白细胞(PMNs)的浸润和基质金属蛋白酶-9(MMP-9)的表达的关系。方法:建立25只家兔角膜碱烧伤模型,分别于伤后3、7、14、21、28d随机处死5只兔,裂隙灯下观察角膜病理修复情况,摘除角膜做病理切片,测定PMNs的浸润量值。免疫组化法测定MMP-9的表达。结果:PMNs和MMP-9在角膜碱烧伤后的3d开始升高,14d上升达到最大峰值,之后逐渐降低,碱烧伤后角膜基质在第14d溃疡面积及深度最为严重。结论:角膜碱烧伤病灶中PMNs和MMP-9的量值呈正相关,且角膜的病理损伤与PMNs的浸润和MMP-9的表达密切相关。

AMI:To study the relationship between the infiltration of polymorphonuclear neutrophils(PMNs)and the expression of matrix metalloproteinases 9(MMP-9)in corneal stroma injury after alkali burn.METHODS:Cornea alkali-burned model was made in 25 rabbits,then animals were grouped and sacrificed at 3d,7d,14d,21d and 28d,respectively.The condition developing of alkali-burned cornea was observed by slit lamp biommicroscopy.The corneas were enucleated for histopathological examination.The infiltration of PMNs was identified by hematoxylin eosin(HE)staining and the expression of MMP-9 was identified by immunohistochemisty in different periods.RESULTS:The quantity of PMNs and MMP-9 increased on the 3d,reached the lower level on 7d,shown a peak on the 14d,then decreased gradually.The area and depth of corneal stroma after alkali burn were the most severe on the 14d.CONCLUSION:During the wound healing process,alkali-burned cornea has close relation with the infiltration of PMNs and the expression of MMP-9.The infiltration of PMNs and the expression of MMP-9 is positively correlated in corneal stroma injury after alkali burn.