翼状胬肉患者眼表微生物菌群分析

Study on the correlation between microbial community on ocular surface and pterygium

目的:研究翼状胬肉患者的眼表微生物菌群组成,探索眼表微生物菌群和翼状胬肉的相关性。方法:收集2018-09/2019-01在我院就诊的原发性翼状胬肉患者,翼状胬肉组为患翼状胬肉的26眼,正常对照组为9只对侧正常眼,采用MiseqPE301+8+8+301平台进行细菌16S rDNA基因V3~V4区双端测序,分析比较翼状胬肉组与正常对照组眼表微生物菌群结构与组成差异。结果:两组样品共得到1 837个OTU;Alpha多样性分析,正常对照组物种的多样性高于翼状胬肉组。同时两组优势菌群相似。构成以棒状杆菌、葡萄球菌、链球菌、丙酸杆菌等革兰氏阳性菌为主,其次为革兰氏阴性菌。棒状杆菌在翼状胬肉组比例增高。结论:翼状胬肉组眼表物种多样性下降,棒状杆菌在翼状胬肉患者眼表菌群的丰度显著增高,可能参与眼表免疫状态调节,并影响翼状胬肉的发生与发展。

AIM:To study the composition of ocular surface microflora in patients with pterygium and to explore the correlation between ocular surface microflora and pterygium.METHODS:Primary pterygium patients were collected from September 2018 to January 2019 in our hospital.The pterygium group consisted of 26 eyes with pterygium and the control group consisted of 9 normal eyes.We used Miseq PE301+8+8+301 platform to measure the sequences of the both ends of bacterial 16S rDNA V3-V4 region.Analyze and compare the structure and composition of microbial flora on normal ocular surface and pterygium ocular surface.WILCOX test(rank sum test)was used for statistical methods.RESULTS:A total of 1 837 OUTs were obtained from the two groups.Alpha diversity analysis showed that the species diversity of the normal control group was higher than that of the pterygium group.At the same time,the dominant flora of the two groups were similar.Corynebacterium,Staphylococcus,Streptococcus,Propionibacterium and other Gram-positive bacteria were the main constituents,followed by Gram-negative bacteria.Corynebacterium increased in pterygium group.CONCLUSION:The diversity of ocular surface species decreased in pterygium group,and the abundance of Corynebacterium in ocular surface flora increased significantly in pterygium patients.Corynebacterium may participate in the regulation of ocular surface immune status,and affect the occurrence and development of pterygium.