高粱糖转运蛋白基因家族全基因组鉴定、分类及表达分析

Genome-Wide Identification, Classification and Expression Analysis of Sugar Transporter Gene Family in Sorghum bicolor

糖转运蛋白(Sugar transporter protein,STP)基因家族在植物单糖分配中具有重要作用,并参与植物生长和发育过程中的许多代谢进程。为探究STP基因家族在高粱生长发育中的潜在功能,对高粱STP基因家族进行了全基因组鉴定、分类和组织表达分析。从高粱基因组中共鉴定出19个包含Sugar_tr结构域的STP基因,不均匀分布于9条染色体上,其中有4个基因位于基因组重复区。19个SbSTP根据其系统发育特征可以分为5组,同组的家族成员具有相同或类似的基序类型和排列顺序,但在内含子和外显子数量上存在较大差异。RNA-Seq数据分析显示,至少有18个SbSTP基因可以表达,不同的基因在不同的组织中具有不同的表达模式,其中SbSTP11特异性在花中表达,SbSTP4、SbSTP5在特定组织中受ABA和PEG的诱导表达。为进一步研究单个SbSTP基因的生物学功能,挖掘SbSTP家族的应用潜力奠定了基础。

The sugar transporter(STP)gene family plays critical roles in monosaccharide distribution and participates in diverse plant metabolic processes.To investigate the potential roles of STPs in sorghum growth and development,the genome-wide identification,classification and expression analyses of the STP gene family were performed in this study.A total of 19 SbSTP genes containing the Sugar_tr conserved motifs were identified from the sorghum genome,which distributed on 9 chromosomes unevenly,and 4 of them located in the genome segmental duplication region.According to their phylogenetic features,the 19 SbSTPs were further classified into 5 main groups,with high similar motif compositions within the same group,but they were different in exon-intron number.The synteny analysis and phylogenetic comparison of STP genes from sorghum,Arabidopsis and rice provided the valuable clues for revealing the evolutionary characteristics of SbSTP genes.RNA-Seq data analysis showed that 18 of the 19 SbSTP genes could expressed in various tissues with distinct expression patterns,among which SbSTP11 exhibited tissue-specific expression in flower,while SbSTP4 and SbSTP5 expressed in root or shoot by induction of ABA and PEG stress.The results provided a valuable resource for better understanding the biological roles and application potentiality of individual STP genes in sorghum.