绿肥套作对植烟土壤微生物群落功能多样性的影响

The Effects of Intercropping Green Manure on Functional Diversity of Microbial Community in Tobacco Planting Soil

以Biolog技术研究了套作绿肥对植烟土壤微生物群落功能多样性的影响,为绿肥与烟草套作栽培模式的应用提供理论依据,结果表明:团棵期和成熟期表征土壤微生物碳代谢活性的平均颜色变化率(AWCD)均为套作草木犀>套作黑麦草>对照,说明套作绿肥对土壤微生物利用碳源的能力影响显著。土壤微生物均匀度指数、Simpson指数和优势度指数均为套作草木犀>套作黑麦草>对照,且套作草木犀的土壤微生物均匀度指数、Simpson指数和优势度指数均为最大值,说明套作绿肥影响了植烟土壤的微生物群落结构,提高了植烟土壤微生物群落多样性。不同碳源的代谢强度分析表明,不同绿肥及不同生长时期土壤微生物对氨基酸类、羧酸类和糖类的利用程度明显高于氨/胺类、其他化合物类和聚合物类碳源。主成分分析表明,套作绿肥及不同生长期植烟土壤的微生物群落功能多样性具有明显的差异,糖类、其他化合物类和羧酸类对微生物碳代谢差异起主要作用。套作绿肥增强了土壤微生物对碳源的利用能力,提高了植烟土壤的微生物群落结构和群落功能多样性,并且套作草木犀的效果更为明显。

The effects of green manure intercropping on the functional diversity of soil microbial communities in tobacco growing soils were studied by Biolog technique to provide the theoretical basis for application of green manure intercropping with tobacco.The average well color development(AWCD)of soil microbial carbon metabolic activity at the stages of rosette and maturity were intercropped with Melilotus officinali>Lolium>control,indicating that intercropping with green manure had a significant effect on soil microbial carbon utilization.The Shannon index,Simpson index and Mclntosh index of intercropping green manure were higher than those of control,while the soil microbial Shannon index,Simpson index and Mclntosh index of intercropping Melilotus officinalis had the highest value.The results showed that intercropping green manure affected the microbial community structure of tobacco growing soil and improved the microbial community diversity of tobacco growing soil.Metabolic intensity analysis of different carbon sources showed that the utilization of amino acid,carboxylic acid and carbohydrate by soil microorganisms in different green manure and different growth stages was significantly higher than that of ammonia/amine,miscellaneou and polymer carbon sources.Principal component analysis showed that the functional diversity of soil microbial community was significantly different,and carboxylic acids,amino acids and sugars were the three main carbon sources,which led to the difference in soil microbial metabolism.Intercropping green manure enhances the ability of soil microorganism to utilize carbon source,and improves the diversity of soil microbial community structure and community function in tobacco growing soils.The effect of intercropping Melilotus officinalis is more obvious.