禽腺病毒4型XZ株增殖与纯化方法的建立

Establishment of the Proliferation and Purification Method of Fowl Adenovirus Serotype 4 XZ Strain

2015年7月以来,禽腺病毒4型在中国的大部分地区暴发流行,并造成重大损失。为进一步对该病毒进行研究,采用两种方法对所分离的XZ株进行体外增殖培养,并利用透射电镜观察到完整的病毒粒子,同时采用超滤浓缩和凝胶层析的方法对病毒进行纯化。结果显示,成功建立了稳定的禽腺病毒4型XZ株增殖体系,以及温和、高效的病毒纯化工艺,且纯化病毒感染性强,具有很好的免疫原性。

Since July 2015,Fowl adenovirus serotype 4 has been epidemic in most parts of China and caused heavy losses.For further study on the virus,two methods were used to proliferate the XZ strain in vitro,the intact virions could be observed by transmission electron microscopy.At the same time,the virus was purified by ultrafiltration and gel chromatography.The results showed that a stable proliferation method of Fowl adenovirus serotype 4 XZ strain and a mild,efficient purification process were established,the purified virus had strong infectivity and good immunogenicity.