恩诺沙星及其代谢物在1日龄鸡体内的药动学研究

Pharmacokinetics of Enrofloxacin and Ciprofloxacin in One-day-old Chicks

研究不同剂量恩诺沙星(ENR)及代谢产物环丙沙星(CIP)在雏鸡体内药动学特征。240只刚出壳黄羽肉鸡分2个剂量组,20 mg/kg和40 mg/kg皮下注射恩诺沙星,高效液相色谱法测定药物浓度,药时数据用WinNolin8.0软件处理非房室模型分析。结果表明,增加剂量后恩诺沙星以下药动参数发生变化:消除半衰期(t 1/2)由7.37 h降至6.53 h,达峰时间(T max)由8 h降到4 h,最大血药浓度(C max)由3.75μg/mL增至4.92μg/mL,药时曲线下面积(AUC last)由40.45 h·μg/mL增至78.2 h·μg/mL,平均滞留时间(MRT last)由为8.6 h增至11.42 h;消除率(Cl)分别为0.49 L/h/kg和0.5 L/h/kg,表观分布容积(Vz obs)分别为5.22 L/kg和4.77 L/kg。不同剂量恩诺沙星给药后都都表现出分布迅速、广泛的特点,代谢产物环丙沙星产生与消除迅速。

In order to study the pharmacokinetics of different doses of enrofloxacin and metabolites in chicks,240 one-day-old chicks were randomly divided into two groups,20 mg/kg group and 40 mg/kg group,and administrated enrofloxacin by subcutaneous injection.The drug concentration was determined by HPLC.The concentration-time data were processed using WinNolin software and analyzed by the non-compartmental model.The main pharmacokinetic parameters of 20 mg/kg group and 40 mg/kg group were as follows,respectively:t 1/2 decreased from 7.37 h to 6.53 h,T max decreased from 8 h to 4 h,C max increased from 3.75μg/mL to 4.92μg/mL,AUC last increased from 40.45 h·μg/mL to 78.2 h·μg/mL,MRT last increased from 8.6 h to 11.42 h,Cl was 0.49 L/h/kg and 0.5 L/h/kg,V z was 5.22 L/kg and 4.77 L/kg.Different doses of enrofloxacin showed rapid and extensive distribution after administration.The metabolite ciprofloxacin was produced and eliminated rapidly.