雄性糖尿病鼠睾丸的组织结构变化和Ghrelin表达

Histological Changes and Ghrelin Expressions in Testes of Male Diabetic Mice

研究糖尿病对小鼠睾丸的组织学影响,检测分析Ghrelin在糖尿病小鼠睾丸内的表达变化特点。通过腹腔注射四氧嘧啶的方法建立糖尿病小鼠模型,分别于造模后第5、10、15天取睾丸组织,采用石蜡切片技术和HE染色方法观察糖尿病小鼠睾丸的组织学变化特点;采用免疫组化染色方法研究糖尿病小鼠睾丸内Ghrelin的表达和分布情况。HE染色结果显示,与对照组相比,随着糖尿病病程的延长,糖尿病小鼠的生精小管内生精细胞数量呈减少趋势,排列层数及紧凑程度呈现不同程度的下降,排列松散无序,并且可见精细胞从基膜脱落。免疫组化结果显示,Ghrelin在对照组小鼠睾丸的初级精母细胞和次级精母细胞的胞膜和胞质内均呈强阳性表达,精原细胞、精子细胞和精子内无阳性表达,支持细胞和睾丸间质细胞的细胞核呈阳性表达。Ghrelin在糖尿病小鼠睾丸内的表达和分布特点与对照组相似,但阳性表达水平较对照组明显增强;随着糖尿病病程的延长,组内Ghrelin表达没有明显变化。试验证实了糖尿病对小鼠睾丸发育和Ghrelin在睾丸内的表达水平均具有一定影响,提示糖尿病对小鼠的生精能力具有一定的抑制作用。

In order to study the histological effects of diabetes on mouse testis,detect and analyze the expression changes of Ghrelin in the testis of diabetic mice,diabetic mouse models were established by intraperitoneal injection of alloxan.Testicular tissue was taken on the 5th,10th,and 15th days after the modelling.The histological changes of the testis of diabetic mice were observed by paraffin section technique and HE staining.The expression and distribution of Ghrelin in the testis of diabetic mice were studied by immunohistochemical staining.The results of HE staining showed that compared with the control group,with the prolongation of diabetes,the number of spermatogenic cells in the seminiferous tubules of diabetic mice decreased,and the number of layers and compactness showed a different degree of decline.And the cells were loosely arranged,it also can be seen that sperm cells are detached from the basement membrane.The results of immunohistochemistry showed that Ghrelin was strongly expressed in the membrane and cytoplasm of primary spermatocytes and secondary spermatocytes in the testis of the control mice,but not in spermatogonia,sperm cells and sperm.Sertoli cells and testicular stromal cells were positive for nuclear expression.The expression and distribution characteristics of Ghrelin in the testis of diabetic mice were similar to those in the control group,but the positive expression level was significantly enhanced compared with the control group.There was no significant change in the expression of Ghrelin in the group as the duration of diabetes progressed.The experiment confirmed that diabetes has a certain effect on mouse testicular development and Ghrelin expression level in the testis,suggesting that diabetes has a certain inhibitory effect on the spermatogenic ability of mice.