Sysmex UF-1000i尿液分析仪细菌通道参数在诊断尿路感染中的应用价值

Application value of bacterial pathway parameters of Sysmex UF-1000i urine analyzer in diagnosing urinary tract infections

目的探讨Sysmex全自动尿沉渣分析仪(UF-1000i)细菌计数在诊断尿路感染(UTI)中的应用价值。方法收集193例尿培养阳性标本并同时作尿沉渣分析,保存尿培养阳性标本的细菌散点图。收集200份健康体检尿标本作UF-1000i尿沉渣分析,记录细菌计数结果。通过受试者工作特征(ROC)曲线计算UF-1000i细菌计数曲线下面积(AUC)和诊断最佳临界点(cut-off)值,并评价其诊断性能。结果UF-1000i细菌计数诊断UTI AUC为0.873,95%CI 0.834~0.912,cut-off值为900个/μL,灵敏度为0.80,特异度为0.94,阳性预测值为0.93,阴性预测值为0.83。细菌散点图显示与细菌培养符合率75.1%。结论UF-1000i用于诊断尿路感染,快速、简便,且具有很好的筛查和确诊价值,避免了不必要的细菌培养,观察的细菌散点图有助于区分球菌或杆菌,为临床早期合理用药提供参考。

Objective To investigate the application value of bacterial count in Sysmex UF-1000i automatic urine analyzer in diagnosing urinary tract infection(UTI).Methods One hundred and ninety-three cases of urine culture positive specimens were collected.The bacterial scatter plot of the urine culture positive specimen was preserved.Two hundreds healthy urine samples were collected for conduct the urine sediment analysis by UF-1000i,and the results of bacteria count were recorded.The receiver operating characteristic(ROC)curve was used to calculate the area under UF-1000i bacterial count curve(AUC)and the cut-off value.Then the diagnostic performance was evaluated.Results AUC of UF-1000i bacterial count for diagnosing UTI was 0.873 with 95%confidence interval(CI)of 0.834-0.912,the cut-off value 900/μL,the sensitivity,specificity,positive predictive value and negative predictive value were 0.80,0.94,0.93 and 0.83 respectively.The coincidence rate between bacterial scatter plot and bacterial culture was 75.1%.Conclusion Using the UF-1000i for diagnosis of urinary tract infection is rapid and simple,has the good value of screening and diagnosis,avoids unnecessary bacterial culture.Observing the bacterial scatter plot help to distinguish cocci from bacilli,which provides reference for early rational use of drugs in clinic.