水通道蛋白1和肿瘤坏死因子受体相关蛋白1在人脑胶质瘤中的表达及相关性

Expression and correlation of AQP1 and TRAP1 in human glioma

目的研究水通道蛋白1(AQP1)和肿瘤坏死因子受体相关蛋白1(TRAP1)在脑胶质瘤中的表达及其之间的相关性。方法选取66例人脑胶质瘤和15例正常脑组织标本,经Western blotting及逆转录聚合酶反应(RT-PCR)对其AQP1、TRAP1 mRNA及蛋白表达进行检测分析。结果①在人脑胶质瘤中AQP1、TRAP1 mRNA及蛋白表达水平较对照组上调(P<0.05);②在高级别胶质瘤(Ⅲ、Ⅳ级)中AQP1、TRAP1 mRNA及蛋白表达与低级别胶质瘤(Ⅰ、Ⅱ级)比较,前者表达水平上调(P<0.05);③AQP1、TRAP1 mRNA及蛋白在脑胶质瘤的表达无相关性(rs=0.074和0.058,P=0.065和0.080)。结论AQP1和TRAP1表达与脑胶质瘤的肿瘤分期有关,恶性程度越高其表达量也越高,但两者与脑胶质瘤的病理进展无协同性。

Objective To investigate the correlation and expression of aquaporin 1(AQP1) and tumor necrosis factor receptor-associated protein 1(TRAP1) in human gliomas. Methods The expression of AQP1 and TRAP1 mRNA and protein were detected by Western blotting and reverse transcription-polymerase reaction(RT-PCR) in 66 human glioma specimens and 15 normal brain specimens. Results ① Compared with the control group, the mRNA and protein expression of AQP1 and TRAP1 in human gliomas were increased(P < 0.05);② Compared with lowgrade glioma(Grade Ⅰ-Ⅱ), the expression of mRNA and protein of AQP1 and TRAP1 in high-grade gliomas(Ⅲ-Ⅳ) increased(P < 0.05);③ There was no correlation between the expression of mRNA or protein of AQP1 and TRAP1 in glioma tissues(r = 0.074 and 0.058, P = 0.065 and 0.080). Conclusions The expression of mRNA and protein of AQP1 and TRAP1 are closely related to the pathological grade of glioma. Higher the degree of malignancy is, more significant the mRNA and protein are;the expressions of AQP1 and TRAP1 have no correlation with the pathological development of glioma.