绿色荧光蛋白标记植物乳杆菌及其生物学特性分析

Biological properties of green fluorescent protein labelled Lactobacillus plantarum

为探究绿色荧光蛋白(green fluorescent protein,GFP)标记对植物乳杆菌生物学特性的影响,采用GFP对4株植物乳杆菌进行标记,并比较标记前后菌株的生物学特性。结果显示,标记前后菌株生长情况基本相同,其中P2和P4被GFP标记后生长略有滞后;标记前后的菌株对胃酸和胆盐的耐受趋势基本一致,无显著差别;在-80℃冻存2周内,标记前后所有菌株的活菌数无显著变化;对低聚果糖利用能力无变化;标记后所有菌株对氯霉素有抗性,标记前菌株对氯霉素敏感,对其他13种抗生素的敏感性无变化。说明GFP标记不影响植物乳杆菌的生物学特性,为可视化研究植物乳杆菌在小鼠肠道内的定植和分布提供了理论依据。

The purpose of this study is to explore whether green fluorescent protein(GFP)labelling has effects on the biological characteristics of Lactobacillus plantarum.Four strains of L.plantarum(P1-P4)were labeled with GFP and their biological characteristics were examined.The results showed that the growth curves of the strains were nearly coincident with and without labeling,although strain P2 and P4 showed slight delay compared to pre-labeled strains.All strains showed no significant differences in the tolerances of gastric acid and bile salts.After being stored at-80°C for two weeks,their viable counts and the ability to utilize fructooligosaccharides did not change significantly.Although their sensitivity to 13 antibiotics were not different,non-labeled strains were sensitive to chloramphenicol but labeled strains not.In conclusion,GFP labelling had no significant effects on the biological characteristics of L.plantarum,which provides a theoretical basis for visualizing the colonization and distribution of L.plantarum in the intestine of mice in future studies.