基于SSR荧光标记进行酒花品种鉴定

Identification of hop varieties based on fluorescent-labeled simple sequence repeats

该研究利用SSR(simple sequence repeats,简单重复序列)荧光标记技术构建酒花品种指纹数据库,目的是建立一种快速、准确的酒花品种真实性鉴定技术。TP-M13-SSR是将M13尾巴序列作为通用接头引入SSR正向引物中,仅对M13通用引物进行荧光标记,结合毛细管电泳对不同引物的扩增产物进行检测。该研究从103对候选引物中筛选到多态性丰富、引物组合区分能力最佳的4对引物,在18个酒花品种中共扩增出28个等位基因,每对引物的等位基因数量3~11个不等,平均每对引物产生7.0个等位基因。通过4对引物可实现18个国内外酒花品种的真实性鉴定,方法快速、准确,可为酒花种植、加工、采购及新品种产权保护方面提供品种真实性鉴定的技术支持。

To establish a rapid and accurate authenticity identification technology,simple sequence repeats(SSR)fluorescent labeling technology was applied in constructing the fingerprint database of hop varieties.In TP-M13-SSR,M13 tail sequence was used as a universal adaptor to the SSR forward primer,only the M13 universal primer was fluorescently labeled,and the amplification products of different primers were detected by capillary electrophoresis.Four pairs of primers with the best polymorphism and combination of primers were screened from 103 candidates.A total of 28 alleles were detected in 18 hop varieties,and each pair of primers produced 3-11 allele fragments with an average of 7.0.The authenticity identification of 18 domestic and international hop varieties could be achieved by the 4 pairs of primers.The method is fast and accurate,which can provide a technical support for hop cultivation,processing and procurement,as well as protection of new varieties.