魔芋葡甘聚糖-海藻酸钠复合益生菌微胶囊的构建及性能评价

Construction and performance evaluation of sodium alginate and konjac glucomannan microcapsules of multi-probiotics

为提高双歧杆菌、植物乳杆菌、干酪乳杆菌3种复合益生菌经胃肠道后的存活率,采用内源乳化法,将菌株包埋在魔芋葡甘聚糖(konjac glucomannan,KGM)和海藻酸钠(sodium alginate,ALG)中制成微胶囊,通过正交实验对工艺参数进行优化,并对其性能进行检测。制备微胶囊的最佳工艺参数:ALG质量分数为3%,KGM质量分数为0.6%,水油体积比为1∶3,CaCO 3与ALG质量比为1∶3,包埋率达到(76.2±5.1)%。微胶囊提高了益生菌在模拟胃液中的存活率,增强了对胆盐的耐受性。通过血清生化指标和组织病理学观察,该材料对小鼠无毒性。灌胃KGM后,小鼠肠道乳杆菌科相对丰度升高,脱硫弧菌科等有害菌相对丰度降低,说明KGM能促进肠道有益菌生长,抑制有害菌。该研究为KGM的应用探索了一条新途径,并为复合益生菌微胶囊产业化生产及推广应用提供参考。

In order to improve the survival rates of Bifidobacteria,Lactobacillus plantarum and Lactobacillus casei after passing through the gastrointestinal tract,these strains were embedded in the microcapsules prepared by internal emulsification with konjac glucomannan(KGM)and sodium alginate.The optimal technological parameters for preparing the microcapsules were as follows:3%ALG(w/w),0.6%KGM(w/w),the volume ratio of water to oil was 1∶3,and the mass ratio of CaCO 3 to ALG was 1∶3.Under this condition,the embedding rate reached(76.2±5.1)%.Moreover,the microcapsules not only improved the survival rate of probiotics in simulated gastric juice,but also enhanced their tolerances to bile salts.The results of serum biochemical parameters and histopathological observation showed that the material was not toxic to mice.After intragastric administration of KGM,the relative abundances of Lactobacillaceae sp.increased and Desulfovibrionaceace sp.decreased,which indicated that KGM could promote the growth of beneficial bacteria and inhibit harmful bacteria.This research expanded a new field for the application of KGM,and the results provide a reference for industrial production and application of compound probiotic microencapsulation.