乳酸发酵拐枣汁对果蝇寿命及抗氧化活性的影响

Effects of Lactobacillus fermented Hovenia dulcis juice on life-span and antioxidant activity of Drosophila melanogaster

为探究活菌型及灭菌型发酵拐枣汁抗衰老、抗氧化能力强弱,以果蝇为试验模型,未发酵拐枣汁做对比,利用各项指标对发酵拐枣汁的体内外抗氧化能力、果蝇寿命的影响进行综合分析。结果表明,随着活菌型及灭菌型发酵拐枣汁饲喂剂量的不断增大,雌雄果蝇的半数死亡时间(median lethal time,LT 50)、平均寿命(mean life-span,MLS)及生存率均不断提高。当饲喂剂量为15 g/L时,与CK组相比,活菌型将雌雄果蝇的平均寿命分别延长了66.49%和61.16%,灭菌型分别延长48.87%和47.69%;用活菌型饲喂20 d的雌雄果蝇体内总超氧化物歧化酶酶活力分别为429.85、431.53 U/mg蛋白,用灭菌型饲喂则分别为390.81、397.77 U/mg蛋白。研究表明,乳酸菌发酵拐枣汁具有抗氧化、延长果蝇寿命、提高果蝇存活率的作用,为发酵拐枣汁的实际产品开发提供理论基础。

In order to investigate the anti-aging and anti-oxidant ability of lively and sterilized fermented Hovenia dulcis juices,the effects of fermented Hovenia dulcis juices on the in vivo and in vitro antioxidant capacity and the life span of Drosophila melanogaster were comprehensively analyzed.The results showed that the median lethal time(LT 50),mean life-span(MLS)and survival rate of male and female Drosophila melanogaster increased with increasing feeding dose of lively and sterilized fermented juices.When fed with 15 mg/mL lively fermented juice,the MLS of male and female Drosophila melanogaster was prolonged by 66.49%and 61.16%respectively,compared with CK group,and sterilized fermented juice fed group was prolonged by 48.87%and 47.69%,respectively.The activities of T-SOD in male and female Drosophila melanogaster fed with lively fermented juice for 20 d were 429.85 and 431.53 U/mg prot,respectively,while that of male and female Drosophila melanogaster fed with sterilized fermented juice were 390.81 and 397.77 U/mg prot,respectively.In conclusion,Lactobacillus fermented juice was anti-oxidative,and could prolong the life span and improve the survival rate of Drosophila melanogaster,which provides a theoretical basis for developing products of fermented Hovenia dulcis juice.