雪上一枝蒿与金不换不同比例配伍的急性毒性研究

Acute Toxicity Study on the Compatibility of Veratrilla baillonii Franch and Aconitum brachypodum Diels

目的:研究金不换对雪上一枝蒿解毒作用并评价二者配伍前后的安全性。方法:采用改良寇氏法测雪上一枝蒿与金不换不同比例合煎配伍的急性毒性,采用上下法测雪上一枝蒿与金不换不同比例合并配伍的急性毒性,并用HE染色法研究心、肝、脾、肺、肾各脏器损伤情况。结果:雪上一枝蒿与金不换合煎配伍5∶1、2∶1、1∶1、1∶2、1∶5测算出的LD50分别为526.38、1047.48、1437.05、1242.37、2280.51 mg·kg^-1,雪上一枝蒿与金不换合并配伍5∶1、2∶1、1∶2、1∶5测算出的LD50分别为55.00、155.77、162.71、174.98 mg·kg^-1。HE染色结果显示死亡小鼠各脏器均无明显病变。结论:雪上一枝蒿与金不换无论合并还是合煎配伍都降低了对小鼠的急性毒性,并且金不换比例越高解毒效果越好。

Objective:To explore the detoxification effect of Veratrilla baillonii Franch to Aconitum brachypodum Diels and evaluate the safety of the two before and after the compatibility.Methods:Karber test was used to measure the acute toxicity of the co-decoction of Veratrilla baillonii Franch and Aconitum brachypodum Diels,up-and-down procedure was used to measure the acute toxicity of the mixed decoction of Veratrilla baillonii Franch and Aconitum brachypodum Diels,HE staining was used to study the damagement of Heart,liver,spleen,lung kidney and stomach.Results:The LD50of co-decoction of Veratrilla baillonii Franch and Aconitum brachypodum Diels 5∶1、2∶1、1∶1、1∶2、1∶5 are 526.38、1047.48、1437.05、1242.37 and 2280.51 mg·kg^-1,The LD50 of the mixed decoction of Veratrilla baillonii Franch and Aconitum brachypodum Diels 5∶1、2∶1、1∶2、1∶5 are 55.00、155.77、162.71 and 174.98 mg·kg^-1,HE staining results showed that there were no obvious lesions in all organs of the dead mice.Conclusion:Whether mixed decoction or co-decoction all reduced the acute toxicity to mice,and the higher the ratio of Veratrilla baillonii Franch,the better the detoxification effect.