苯扎贝特和非诺贝特对肺腺癌PC-9细胞增殖和c-myc表达的影响

Effects of Bezafibrate and Fenofibrate on the Proliferation of Lung Adenocarcinoma PC-9 Cells and the Expression of c-myc

目的:研究贝特类药物苯扎贝特和非诺贝特对肺腺癌PC-9细胞增殖和c-myc表达的影响。方法:采用CCK8法检测苯扎贝特和非诺贝特(12.5、25、50、100、20μmol/L)作用48 h对PC-9细胞存活率的影响。另将PC-9细胞分为给药组和对照组,给药组分别加入低、中、高浓度(25、50、100μmol/L)的苯扎贝特和非诺贝特,对照组加入二甲基亚砜,作用48 h后,采用流式细胞仪检测细胞周期分布及凋亡情况;荧光定量聚合酶链法(qRT-PCR)检测细胞中c-myc mRNA相对表达量;Western blot法检测细胞中c-myc蛋白相对表达量。结果:上述浓度苯扎贝特作用下PC-9细胞存活率分别为(80.76±3.2)%、(74.35±5.06)%、(62.8±1.23)%、(59.03±1.55)%、(39.8±1.01)%;而非诺贝特作用下PC-9细胞存活率分别为(74.46±1.30)%、(61.91±4.77)%、(48.95±2.8)%、(37.05±1.55)%、(32.49±1.36)%。与对照组比较,苯扎贝特和非诺贝特的中、高浓度组G1期细胞比率均明显增加,苯扎贝特和非诺贝特的低、中、高浓度组的细胞凋亡率均明显增加,c-myc mRNA和蛋白相对表达量均明显降低,差异具有统计学意义(P<0.05)。结论:苯扎贝特和非诺贝特可抑制PC-9细胞增殖,并下调c-myc表达。

OBJECTIVE:To study the effects of bezafibrate(BEZ)and fenofibrate(FEN)on the proliferation of lung adenocarcinoma PC-9 cells and the expression of c-myc.METHODS:The effects of BEZ and FEN(12.5,25,50,100,200μmol/L)on the survival rate of PC-9 cells were detected by CCK8 method.PC-9 cells were divided into administration group and control group.Administration group was given low,medium and high concentration(25,50,100μmol/L)of BEZ and FEN;control group was treated with dimethyl sulfoxide for 48 h.Cell cycle distribution and apoptosis were detected by flow cytometry.qRT-PCR was used to detect m RNA relative expression of c-myc in cells.The protein relative expression of c-myc in cells were detected by Western blot assay.RESULTS:The survival rates of PC-9 cells were(80.76±3.2)%,(74.35±5.06)%,(62.8±1.23)%,(59.03±1.55)%,(39.8±1.01)%under the action of above concentration of BEZ;and the survival rates of PC-9 cells were(74.46±1.30)%,(61.91±4.77)%,(48.95±2.8)%,(37.05±1.55)%,(32.49±1.36)%under the action of FEN.Compared with control group,G1 phase cell ratio increased significantly in medium and high concentration groups of BEZ and FEN;the apoptotic rate of PC-9 cells was increased significantly in low,medium and high concentration groups of BEZ and FEN;mRNA and protein relative expression of c-myc were decreased significantly,with statistical significance(P<0.05).CONCLUSIONS:BEZ and FEN can inhibit the proliferation of PC-9 cells,and down-regulate c-myc expression.