槐白皮水提物对免疫抑制小鼠的免疫作用

Effects of Sophora Japonica Water Extract on the Immune Functions of Immunosuppressive Mice

目的探讨槐白皮水提物对免疫抑制小鼠免疫功能的影响。方法昆明小鼠随机分为正常组,模型组,香菇多糖组和槐白皮水提物低、中、高剂量组,除正常组外,其余各组连续3d腹腔注射环磷酰胺复制免疫抑制模型。造模后连续灌胃给药14 d,血细胞计数仪测定小鼠外周血白细胞数目、称量并计算免疫器官系数、MTT法测定脾淋巴细胞增殖和NK细胞活性,ELISA检测血清IL-2、IFN-γ和TNF-α水平。结果槐白皮水提物中、高剂量组明显提高免疫抑制小鼠的外周血白细胞水平和免疫器官系数,显著增加LPS诱导的脾淋巴细胞增殖,显著增强脾NK细胞活性,提高免疫抑制小鼠血清IL-2、IFN-γ水平;槐白皮水提物高剂量组明显提高ConA诱导的脾淋巴细胞增殖,显著提高免疫抑制小鼠血清TNF-α水平。结论槐白皮水提物可以提高免疫抑制小鼠的免疫功能。

OBJECTIVE To explore the effects of Sophora japonica water extract on the immune functions of immunosuppressive mice. METHODS Kunming mice were divided into normal group, model group, lentinus edodes polysaccharide group and low-, moderate-, high-dose Sophora japonica water extract group. The immunosuppressive mice model was produced by intraperitoneal injection of cyclophosphamide for there consecutive 3 d in each group except the normal group. After 14 d of continuous intragastric administration, white blood cells number, spleen index and thymus index were measured to evaluate the immunomodulatory effect of Sophora japonica water extract. In addition, spleen lymphocyte proliferation and NK cell activity were assayed by MTT, the levels of IL-2, IFN-γ and TNF-α in serum were measured by ELISA. RESULTS Sophora japonica water extract moderate-and high-dose groups obviously elevated the peripheral white blood cells and the immune organ coefficient, significantly increased the proliferation of spleen lymphocyte caused by LPS, obviously elevated the NK cell activity and levels of IL-2 and IFN-γ in serum of immunosuppressive mice. Sophora japonica water extract high-dose group obviously elevated the proliferation of spleen lymphocytes caused by ConA and the level of TNF-α in serum. CONCLUSION Sophora japonica water extract can enhance the immune function of immunosuppressive mice.