建兰炭疽病拮抗放线菌的筛选及防治效果

Screening and Effect of Antagonistic Actinomycetes against Anthracnose of Cymbidium ensifolium

为筛选获得对建兰炭疽病菌胶孢炭疽菌具有良好抑菌效果的放线菌,以野生山地兰花表层土壤为分离样本,从土壤样品中分离获得25株放线菌,初筛出6株对建兰炭疽病菌胶孢炭疽菌具有明显抑制作用的菌株,其中菌株SVFJ-07的抑菌效果最好,抑菌率达83.64%。经形态特征、生理生化特征及16S rDNA序列分析,确认菌株SVFJ-07为酒红链霉菌。该菌对多种真菌具有良好的抑菌效果。对建兰炭疽病的室内防效为70.06%,田间防效66.27%~68.54%。可见,酒红链霉菌SVFJ-07对建兰炭疽病菌胶孢炭疽菌具有较好的拮抗作用,生防应用前景良好。

In order to screen the actinomycetes with good antifungal effect against the anthracnose pathogen on Cymbidium ensifolium,actinomycetes were isolated from surface soil sample of wild mountain orchid.The results showed that a total of 25 strains of actinomycetes were isolated,and 6 strains had obvious inhibitory effect on Colletotrichum gloeosporioides of C.ensifolium.Strain SVFJ-07 had the best effect with the inhibitory rate of 83.64%.Based on morphological,physiological and biochemical characteristics and 16S rDNA sequence analysis,the strain SVFJ-07 was identified as Streptomyces vinaceus.It had good inhibition effect on several fungi.The sterile fermentation broth could inhibit C.ensifolium of the anthracnose by 70.06%and 66.27%—68.54%in the lab and in the field,respectively.All the results indicated that strain SVFJ-07 was effective to control C.gloeosporioides of C.ensifolium,and had promising application prospect in biological control.