基于聚集诱导发光的纳米粒子用于肝癌细胞靶向成像

Specific Hepatocellular Carcinoma Imaging Based on Aggregation-Induced Emission Nanoparticles

利用具有聚集诱导发光特性的荧光染料4,4'-[(1E,1'E)蒽-9,10-二基双(乙烯-2,1-二基)]双(N,N-二甲基苯胺)(NDSA),通过两亲性聚合物二硬脂酰基磷脂酰乙醇胺-聚乙二醇-N-羟基琥珀酰亚胺(DSPE-PEGNHS)包覆的方法制备了明亮的橙色荧光纳米粒子,其最大发射波长为559 nm,在水溶液中具有2. 89%的荧光量子产率.该纳米粒子具有优异的发光特性和良好的生物相容性.在该纳米粒子表面修饰肝癌细胞靶向的人类婆罗双树样基因-4(SALL4)抗体后,荧光纳米粒子NDSA@SALL4可以特异性地靶向肝癌细胞,还可以在细胞核富集,呈现出明亮的橙色荧光,为早期检测肝癌细胞提供了可能.

A bright orange fluorescent nanoparticles NDSA@SALL4[NDSA=4,4′ (1E,1′E) 2,2 (anthra cene 9,10 diyl)bis(ethane 2,1 diyl)bis(N,N dimethylaniline),SALL4=Sal like protein 4]encapsulated by amphiphilic polymer 1,2 distearoyl sn glycero 3 phosphoethanolamine N [succinimidyl(polyethylene glycol)](DSPE PEG NHS)was prepared,which further modified SALL4 antibody.The nanoparticles NDSA@SALL4 shows good biocompatibility and high photoluminescence,which peaked at 559 nm and exhibited a fluorescence quantum yield of 2.89% in aqueous medium.NDSA@SALL4 can not only specifically target liver cancer cells,but also accurately image the nucleus of cell with bright orange fluorescence,demonstrating that they have a good candidate for early diagnose and bioimaging applications.