不同配伍比例栀子-肉桂水提物改善小鼠睡眠作用及相关机制研究

Effects of Different Proportions of Gardenia and Cinnamon cassia Aqueous Extract on Sleep Function and Related Mechanisms in Mice

研究不同质量比例栀子-肉桂水提物改善睡眠作用,初步探讨改善睡眠的作用机制。选取150只18 g^22 g健康雄性成年昆明小鼠,按体重对实验动物随机分组,每组10只,每天经口灌胃给予阴性对照组(生理盐水)、按质量比10∶1、5∶1、1∶1栀子-肉桂水提物组、阳性对照组(地西泮),连续灌胃受试样品30 d后,进行直接睡眠实验、延长戊巴比妥钠睡眠实验、戊巴比妥钠阀下剂量催眠实验、巴比妥钠睡眠潜伏期实验;采用酶联免疫吸附(enzyme linked immunosorbent assay,ELISA)法测定不同质量比例小鼠脑5-羟色胺(5-hydroxytryptamine,5-HT)、γ-氨基丁酸(γ-aminobutyric acid,GABA)的含量。结果表明,栀子-肉桂不同质量比例组在60 min内,均未有直接睡眠现象;与阴性对照组相比,栀子-肉桂质量比5∶1组能延长戊巴比妥钠小鼠睡眠时间(P<0.05),且能增加戊巴比妥钠阀下剂量小鼠入睡率(P<0.05);栀子-肉桂质量比10∶1组能缩短巴比妥钠小鼠睡眠潜伏期(P<0.05);栀子-肉桂质量比5∶1组能增加小鼠脑内的GABA含量(P<0.05),栀子-肉桂不同质量比例组对小鼠脑内5-HT含量无明显影响(P>0.05)。表明栀子-肉桂质量比5∶1组改善睡眠效果最佳,其作用机制可能与增加小鼠脑内GABA含量有关。

The effect of different proportion of gardenia-Cinnamon cassia aqueous extract and the mechanism of the optimal proportion on improving sleep were studied.A total of 150 healthy adult Kunming male mice ranging from 18 g to 22 g were selected and randomly divided into experimental groups according in line with their weights,10 in each group.Each day,gardenia-Cinnamon cassia aqueous extract of different proportion was given by oral intragastric administration with low,high dose,negative control group(normal saline);according to the mass ratio of 10∶1,5∶1 and 1∶1 gardenia-Cinnamon cassia aqueous extract group;and the positive control group of western medicine(diazepam).After continuous intragastric administration for 30 days,the sleep test,the prolonged sleep test with sodium pentobarbital,hypnosis test with sodium pentobarbital under valve and the sleeping incubation period induced by barbital sodium experiment were performed directly.The contents of 5-hydroxytryptamine(5-HT)andγ-aminobutyric acid(GABA)in the brain of mice were deter-mined by enzyme linked immunosorbent assay(ELISA).The resulets showed that,varied compatibility propor-tion of gardenia and Cinnamon cassia had no direct sleep within 60 minutes;compared with the negative control group,The gardenia-Cinnamon cassia mass ratio 5∶1 group could prolong the sleep time of pentobarbital sodium mice(P<0.05),and increase the sleep rate of pentobarbital sodium valve mice(P<0.05).Gardenia-Cinnamon cassia mass ratio10:1 group can shorten the sleep latency of barbiturate mice(P<0.05).Compared with negative control group,the gardenia-Cinnamon cassia mass ratio 5∶1 group could significantly increase the content of GABA in mice brain(P<0.05),but had no evident effect on the content of 5-HT(P>0.05).The gardenia-Cinnamon cassia mass ratio 5∶1 group generated the best effect on improving sleep,and its mecha-nism might be related to the increase of GABA content in the brain of mice.