乙醛脱氢酶1在乳腺癌转移淋巴结中的表达及其临床意义

Expression of aldehyde dehydrogenase 1 in metastatic lymph node of breast cancer and its clinical significance

目的探讨乙醛脱氢酶1(ALDH1)在乳腺癌转移淋巴结中的表达及其临床意义。方法收集2012年2月至2014年1月在广州市红十字会医院行乳腺癌手术患者的临床资料,采用免疫组织化学染色法检测乳腺癌转移淋巴结(n=47)和未转移淋巴结(n=15)中ALDH1的表达,分析转移淋巴结中ALDH1表达与乳腺癌患者临床病理特征及预后的关系。结果乳腺癌转移淋巴结和未转移淋巴结中ALDH1阳性表达率分别为36.17%(17/47)、6.67%(1/15),转移淋巴结中ALDH1阳性表达率显著高于未转移淋巴结(χ^2=4.804,P<0.05)。乳腺癌转移淋巴结中ALDH1表达与患者肿瘤临床分期、肿瘤直径、雌激素受体、孕激素受体、人类表皮生长因子受体2、Ki-67表达及原发灶分子分型无关(P>0.05),与肿瘤分级及淋巴结转移数有关(P<0.05)。乳腺癌转移淋巴结ALDH1表达阳性患者和ALDH1表达阴性患者5 a生存率分别为82.4%(14/17)、90.0%(27/30),乳腺癌转移淋巴结ALDH1表达阳性患者与ALDH1表达阴性患者5 a生存率比较差异无统计学意义(χ^2=0.576,P>0.05)。结论乳腺癌转移淋巴结中ALDH1表达与原发灶肿瘤分级、淋巴结转移数相关,但转移淋巴结中ALDH1表达与患者预后无关。

Objective To study the expression of aldehyde dehydrogenase 1(ALDH1)in metastatic lymph nodes of breast cancer and its clinical significance.Methods The clinical data of breast cancer patients who underwent operation in Guangzhou Red Cross Hospital from February 2012 to January 2014 were collected.The expression of ALDH1 in 47 cases of metastatic lymph nodes and 15 cases of non-metastatic lymph nodes was detected by immunehistochemical staining method.The relationship between the expression of ALDH1 and clinic pathological parameters,prognosis of breast cancer patients was analyzed.Results The positive expression rate of ALDH 1 in metastatic and non-metastatic lymph nodes of breast cancer was 36.17%(17/47)and 6.67%(1/15),respectively.The positive expression rate of ALDH1 in metastatic lymph nodes was significantly higher than that in the non-metastatic lymph nodes(χ^2=4.804,P<0.05).The expression of ALDH1 in metastatic lymph nodes of breast cancer was not related to clinical stage,tumor diameter,expression of estrogen receptor,progesterone,Ki-67 and human epidermal growth factor receptor 2 and the molecular typing of primary lesions(P>0.05),but it was related to tumor grade and lymph node metastasis(P<0.05).The 5-year survival rate of patients with positive expression of ALDH1 and negative expression of ALDH1 in metastatic lymph nodes of breast cancer was 82.4%(14/17),90.0%(27/30),respectively;there was no significant difference in 5-year survival rate between patients with positive expression of ALDH1 and negative expression of ALDH1 in metastatic lymph nodes of breast cancer(χ^2=0.576,P>0.05).Conclusion The expression of ALDH1 in metastatic lymph nodes of breast cancer is correlated with the grade of primary tumor and the number of lymph node metastases,but the expression of ALDH1 in metastatic lymph node is not correlated with the prognosis of patient.