胸苷和诺考达唑对胃癌细胞周期同步化的调节作用

The moderating effect of thymidine and nocodazole on gastric cell cycle synchronization

目的观察有丝分裂阻滞剂胸苷和诺考达唑对胃癌MGC-803细胞株细胞周期同步化的调节作用及其形态的影响。方法以2mmol/L的胸苷和50ng/ml的诺考达唑处理细胞,最后在不同时间点收集细胞,并用流式细胞仪检测细胞周期;光镜下观察各组细胞形态变化。结果胸苷处理后,细胞周期被阻滞在G1/S期;50ng/ml诺考达唑处理后,细胞周期均被阻滞在G2/M期;以双胸腺嘧啶苷法处理细胞后,细胞形态无明显变化;以50ng/ml诺考达唑法处理细胞后,细胞形态呈现煎蛋样改变,且细胞形态变大,胞质显著增多,细核呈现多形性。结论胸苷和诺考达唑对胃癌细胞周期同步化具有调节作用,且当药物撤离后,细胞周期可逐渐恢复;胸苷对细胞形态影响较小,而诺考达唑对细胞形态产生显著变化。

Objective To investigate the effect of thymidine and nocodazole on cell cycle synchronization and morphology of gastric cancer cell line MGC-803.Methods The cells treated with 2 mmol/L of thymidine and 50 ng/ml of nocodazole were collected at different time points,and the cell cycle was detected by flow cytometry.Results After treated with thymidine,the cell cycle was blocked in G1/S phase;after treated with 50 ng/ml of nocoidazole,the cell cycle was blocked in G2/M phase;after treated with dipyrimidine,the cell morphology did not change significantly;after treated with 50 ng/ml of nocoidazole,the cell morphology showed egg-like changes,the size of cell become bigger,the cytoplasm increased significantly and the nucleus exhibited polymorphism.Conclusion Thymidine and nocodazole can regulate the synchronization of cell cycle in gastric cancer,and the cell cycle can gradually recover after withdrawal of the drugs.Thymidine has little effect on cell morphology,while nocodazole has significant effects on cell morphology.