基于抗高尿酸血症活性的鸡矢藤物质基础研究

Based on Anti-hyperuricemia of Extracts and Research on Substantial of Paederia Scandens(Lour.) Merr.

目的研究鸡矢藤提取物抗高尿酸作用的药效物质基础。方法次黄嘌呤复制高尿酸小鼠模型,观察鸡矢藤提取物对高尿酸血症小鼠血清尿酸水平的影响;观察对脂多糖诱导的小鼠巨噬细胞RAW264.7的NO水平的影响,对DPPH自由基的清除率。采用HPLC-LTQ/Orbitrap MS法快速鉴定鸡矢藤提取物的化学成分。结果鸡矢藤提取物可显著降低次黄嘌呤诱导的高尿酸血症小鼠血清尿酸水平(P<0.05或P<0.01),可显著降低脂多糖诱导的小鼠巨噬细胞RAW264.7的NO水平(P<0.05或P<0.01),对DPPH自由基清除的IC50为1.4 mg·mL^-1。采用HPLC-LTQ/Orbitrap MS法从鸡矢藤提取物中分析鉴定出14个成分,其中包括京尼平龙胆二糖甙、鸡矢藤次苷甲酯、鸡矢藤苷、京尼平苷、7,8-Dihyroiridoid、鸡矢藤次苷、天竺葵素-3-葡萄糖苷、芦丁、异槲皮素、紫云英苷、飞燕草素、大黄酚、大黄素、绿原酸。结论应用高尿酸小鼠模型及HPLC-LTQ/Orbitrap MS法快速筛选鸡矢藤提取物中可能的活性成分,可为鸡矢藤抗高尿酸药效物质基础研究提供一种快速、高效的方法学借鉴。

OBJECTIVE To study the chemical constituents and the effects on anti-hyperuricemia of extracts from Paederia scandens(Lour.)Merr..METHODS Hypoxanthine was used to replicate hyperuricemia in mice.The serum uric acid level in mice,the level of NO in LPS-induced macrophage RAW264.7 and DPPH free radical scavenging rate of total glycosides from Paederia scandens(Lour.)Merr.were detected.The chemical constituents of the otal glycosides were identified by HPLC-LTQ/Orbitrap MS.RESULTS The extract of Paederia scandens(Lour.)Merr.significantly reduced the serum uric acid level of hypoxanthine-induced hyperuricemia mice(P<0.05 or P<0.01);The NO level in LPS-induced RAW264.7 cells was significantly suppressed by the extract of Paederia scandens(Lour.)Merr.(P<0.05 or P<0.01);The IC50 of DPPH free radical scavenging was 1.4 mg·mL^-1;The chemical constituents of the total glycosides from Paederia scandens(Lour.)Merr.were mainly included genipin-1-β-D-gentiobioside,scandoside methyl ester,paederoside,geniposide,7,8-Dihyroiridoid,scandoside,pelargonidin-3-glucoside,rutin,isoquercitrin,astragalin,delphinidin,chrysophanol,emodin,chlorogenic acid.CONCLUSION HPLC-LTQ/Orbitrap MS method was used for rapid screening of the possible active ingredients in the extracts of Paederia scandens(Lour.)Merr.,which could provide a rapid and efficient methodological reference for the basic research of the anti-hyperuricemia substances of Paederia scandens(Lour.)Merr..