丹参酮ⅡA对酸浆苦素D在大鼠体内药动学特性的影响

Effect of Tanshinone ⅡA on Pharmacokinetic Characteristics of Physalin D in Rats

目的研究丹参酮ⅡA对酸浆苦素D在大鼠体内药动学特性的影响。方法12只SD大鼠随机分为对照组和丹参酮ⅡA组,分别灌胃生理盐水与丹参酮ⅡA,丹参酮ⅡA(50 mg·kg^-1,每天1次)连续给药3 d后,酸浆苦素D灌胃给药后按时间点连续采样,采用HPLC-MS/MS测定酸浆苦素D在大鼠血浆中的含量,采用WinNolin 6.3软件计算主要药动学参数,2组间药动学参数采用SPSS 11.0统计软件对药动学参数进行t检验。结果对照组和丹参酮ⅡA组酸浆苦素D的药动学参数间差异存在显著性差异(P<0.05),连续给药丹参酮ⅡA 3 d后给酸浆苦素D,酸浆苦素D的AUC0-24 h及Cmax均有显著提高。结论丹参酮ⅡA能够显著影响酸浆苦素D在大鼠体内药动学过程。

OBJECTIVE To study the effect of carboxylesterase inhibitor(tanshinoneⅡA)on pharmacokinetic characteristics physalin D in rats.METHODS Twelve SD rats were randomly divided into the control group and tanshinoneⅡA group,which was administrated respectively with normal saline and tanshinoneⅡA.After pretreated with tanshinoneⅡA(50 mg·kg^-1,qd)for 3 d,physalin D was given to the rats by intragastric administration.The plasma concentrations of physalin D were determined by HPLC-MS/MS.The main pharmacokinetic parameters were calculated by WinNonlin 6.3 software,pharmacokinetic parameters between two groups were then compared with t test by SPSS 11.0 software.RESULTS There had significant differences between the two groups in the main pharmacokinetic parameters of physalin D(P<0.05).AUC0-24 h and Cmax of oxymatrine D were significantly increased after continuous administration of tanshinoneⅡA for 3 d.CONCLUSION GinsenoneⅡA can significantly influence the pharmacokinetic process of physalin D in rats.