中华绒螯蟹肝胰腺“白化”症体组织及池塘水体、底泥微生物多样性分析

“Whitening” Disease of Hepatopancreas of Eriocheir sinensis: Microbial Diversity Analysis of Tissue,Pond Water and Sediment

研究肝胰腺“白化”河蟹样品,利用高通量测序技术对发病池塘水体、底泥及发病前后组织样品中微生物多样性进行分析,基于该批次样品分析,蟹体发病前后鳃、肝胰腺和肠道组织的菌群丰度和多样性成反比例关系。外来菌群对蟹体的侵染过程首先在鳃组织,其后为肝胰腺,最后为肠道。发病河蟹肝胰腺柔膜菌纲的菌群占比,较健康个体急剧上升。

To study the changes of microorganisms in various tissues,pond water and sediment in a case of hepatopancreas“whitening”Eriocheir sinensis samples were researched.The high-throughput sequencing technology was used to analyze the microbial diversity in pond water,sediment and tissue samples before and after onset.According to the aes he abundance and diversity of sputum,hepatopancreas and intestinal tissues of crabs were inversely proportional before and after the onset.The process of infection of the crab by the foreign flora was as follows:firstly in the sputum tissue,then by the hepatopancreas,and finally in the intestine.Compared with healthy crabs,the proportion of the flora in Mollicutes of the hepatopancreas“whitening”samples had increased sharply.