嗜酸性氧化亚铁硫杆菌耐氟性研究

Study on Fluoride Resistance of Eosinophilic Thiobacillus Ferrooxidans

氟可以抑制微生物浸出过程中微生物的生长。为探究微生物在不同矿物质的生物浸出过程中呈现不同氟耐受性的原因,利用嗜酸性氧化亚铁硫杆菌在不同生长时期进行氟胁迫试验。结果表明,迟滞期前引入氟化物,F-浓度为0.5和1.0 mmol/L时,Eh、Fe^2+氧化速率及细菌生长活性与对照组相似,F-浓度分别为1.5、3.0和5.0 mmol/L时,Eh和Fe^2+氧化速率明显下降直至停滞,细菌生长活性从受到轻微影响变化到绝对停滞;对数生长期前引入氟化物,氟浓度为10.0 mmol/L时才表现出明显延迟,20.0 mmol/L的氟胁迫时,细菌无法正常生长。

Fluorine can inhibit growth of microorganisms.Fluorine stress test of eosinophilic Thiobacillus Ferrooxidans was carried out at different growth stage to investigate fluoride tolerance of microorganisms.The results show that when fluoride is introduced with F-concentration of 0.5 and 1.0 mmol/L respectively before lag period,Eh,Fe ^ 2+oxidation rate and bacterial growth activity are similar to those of control group.When F-concentration is 1.5,3.0 and 5.0 mmol/L,respectively,Eh and Fe ^ 2+oxidation rate drop significantly until to being stagnant,and bacterial growth activity changes from bering slightly affected to absolute stagnation.When fluoride is introduced before logarithmic phase,bacterium show a significant delay when fluorine concentration is up to 10.0 mmol/L.Bacterium cannot grow normally when fluorine stress is 20.0 mmol/L.