基于系统药理学的骨碎补治疗骨质疏松的通路及靶标探讨

Pathways and targets of Gusuibu in the treatment of osteoporosis based on systematic pharmacology

[目的]通过系统药理学相关生物信息学分析方法探讨中药材骨碎补治疗骨质疏松的的生物学通路及靶标。[方法]通过中药系统药理学分析平台(TCMSP)数据库提取骨碎补的活性成分和有关的靶标蛋白。通过运用软件Cytoscape 3.5.1构筑骨碎补有效成分与靶标关系网络进行拓扑学分析。使用STRING数据库进行蛋白之间相互作用(PPI)网络的构筑与分析。借助David数据库进行KEGG通路功能富集分析。[结果]表明骨碎补的核心化合物有柚皮苷、圣草酚、山奈酚等,重要的靶标有前列腺素G/H合成酶2(Prostaglandin G/H synthase 2)、Heat shock protein HSP 90、Caspase-3等,骨碎补发挥生物学效应作用于骨质疏松症的关键蛋白主要有JUN、TP53、MAPK1、白介素-6(IL-6)、雌激素受体1(ESR1)等。KEGG结果表明骨碎补主要作用于TNF signaling pathway、T cell receptor signaling pathway、HIF-1 signaling pathway、PI3K-Akt signaling pathway、Estrogen signaling pathway等相关信号通路。[结论]骨碎补主要通过多种途径,多种信号通路作用于多种靶点发挥抗骨质疏松的生物学效用。

[Objective]To explore the biological pathway and target of gusuibu in the treatment of osteoporosis by systematic pharmacology-related bioinformatics analysis.[Methods]The active constituents and target proteins of Gusuibu were extracted from TCMSP database.By using Cytoscape 3.5.1 to construct the relationship network between effective components and target of gusuibu for topological analysis.STRING10.5 database was used to construct and analyze protein-protein interaction(PPI)network.KEGG pathway function enrichment analysis was carried out with the help of David database.[Results]Naringin,eriodic tyol and kaempferol were the core compounds of Gusuibu.Prostaglandin G/H synthase 2,Heat shock protein HSP 90 and Caspase-3 were the important targets.JUN,TP53,MAPK1,IL-6,ESR1 were the key proteins in the effective components of gusuibu.KEGG results showed that osteoporosis mainly acted on TNF signaling pathway,T cell receptor signaling pathway,HIF-1 signaling pathway,PI3 K-Akt signaling pathway,estrogen signaling pathway and other related signaling pathways.[Conclusion]Gusuibu has the biological effect of anti-osteoporosis through a variety of pathways and signaling pathways acting on a variety of targets.