并殖吸虫DNA分类技术的研究进展

Progress in the identification of Paragonimus species by DNA technology

并殖吸虫病是重要的食源性寄生虫病,其病原体通过中间宿主溪蟹、蜊蛄等传播,可寄生于人体及多种哺乳动物组织、脏器内,引起并殖吸虫病。我国是并殖吸虫分布最广且种类最多的国家,27个省(直辖市、自治区)均有并殖吸虫感染病例的报告。近年来,随着分子生物学技术的发展,特别是DNA技术的进步,对并殖吸虫的分类和鉴定发挥了巨大的作用。不同DNA检测技术的敏感性、特异性、检测时间和检测成本存在差异,需要根据不同的需求选择相应的方法。本文综述了国内外并殖吸虫DNA分类技术研究进展,以期为并殖吸虫的分类鉴定提供参考。

Paragonimiasis is an important food-borne parasitic disease caused by the infection of Paragonimus spp.through ingestion of the intermediate host crabs,crayfishes contaminated with Paragonimus larvae.The tremotode parasitizes in different tissues and organs of human and other mammals and cause paragonimiasis.China is the country with the most widespread of the disease and diverse species of Paragonimus.Paragonimus infections have been reported in 27 provinces or regions in China.In recent years,the development of molecular biology technology including DNA detection technique has played an important role in the identification of Paragonimus species and strains.However,different DNA detection assays own different detection sensitivity,specificity,detection time and cost.The appropriate DNA detection method should be selected according to the different needs.In this paper,the research progress in the identification of Paragonimus species and strains by the DNA detection assays developed by domestic and international scientists is reviewed in order to provide reference for accelerating the identification Paragonimus species and the diagnosis of paragonimiasis.