杜仲脂氧合酶基因家族全基因组鉴定及其表达特性研究

Genome-wide Identification and Expression Characteristics of LOX Gene Family in Eucommia ulmoides

LOX(脂氧合酶)是动、植物中普遍存在的一种氧合酶,在植物的生长发育和逆境胁迫中发挥重要作用。本研究在杜仲基因组范围内,利用生物信息学方法对LOX基因家族进行全面鉴定,并分析了其基因结构、定位及其所编码蛋白质的理化性质、结构特征、分类和功能等。结果显示:杜仲LOX基因家族有23条LOX基因序列,分别定位于细胞质或叶绿体中,分为9-LOX和13-LOX两个亚家族,其中9-LOX类型仅包含一个,13-LOX类型包含22个;23个LOX基因非平均分布于12条scaffolds上,一些基因发生串联分布;RNA-Seq表达结果显示6个13-LOX类型基因在杜仲叶、果、树皮和种仁中几乎没有表达,而其他17个LOX基因表达具有组织和时空表达特异性。研究结果为揭示杜仲LOX基因家族成员的功能提供重要线索,为进一步研究LOX基因家族提供理论基础。

LOX(lipoxygenase)is a kind of oxygenase widely distributed in plants and animals,which are involved extensively in plant growth,development and abiotic and biotic stress.A genomed-wide search was carried out to identify LOX genes in Eucommia ulmoides,we also analyzed the LOX genes structure,localization,and chemicophysical properties,classification and function of protein by bioinformatics method.The E.ulmoides genome contained 23 putative LOX genes clustered into two groups,designated 9-LOX and 13-LOX,but only one LOX belonged to 9-LOX,and other 22 LOX belonged to 13-LOX,were targeted to cytoplasm or chloroplast.These LOX genes were located on the 12 scaffolds in nonrandom ways,mostly grouped together within single scaffold.RNA-Seq showed 613-LOX genes were not expressed in bark,fruit,leaf and kernel,while the expression of other 17 LOX genes were different in four organs from different developmental stages.The results of this investigation could definitely provide a significant foundation for further research on function analysis of E.ulmoides LOX gene family.