山西黄芪根际土壤中丛枝菌根真菌的初步分离与鉴定

Preliminary separation and identification of arbuscular mycorrhizal fungi from rhizosphere soil of Astragalus membranaceus in Shanxi

目的:对山西黄芪根际土壤中存在的丛枝菌根真菌孢子进行分离及鉴定。方法:在山西黄芪根际30 cm土壤范围内,按0~10 cm、10~20 cm、20~30 cm垂直分层采集土样,采用湿筛倾析-蔗糖离心法分离丛枝菌根真菌孢子,利用形态特征对分离孢子进行鉴定;采用碱解离-乳酸甘油酸性品红染色法研究丛枝菌根真菌对黄芪根系的侵染率。结果:从山西浑源3年野生蒙古黄芪根际土壤中共分离3属12种丛枝菌根真菌孢子,根系平均侵染率为33.4%;从山西中医药大学校园3年生栽培膜荚黄芪根际土壤中共分离出3属6种丛枝菌根真菌孢子,根系平均侵染率为9.6%。结论:不同产地黄芪根际土壤中的丛枝菌根真菌孢子种类和根系平均侵染率不同。

Objective:To separate and identify the arbuscular mycorrhizal fungi spores from rhizosphere soil of Astragalus membranaceus.Methods:Within the 30 cm soil of rhizosphere,the soil samples were collected from 0~10 cm,10~20 cm,20~30 cm vertically.The mycorrhizal fungal spores were isolated by wet-screening and sucrose-centrifugation method and identified by morphological features.The infection rate of mycorrhizal fungi on root system of Astragalus membranaceus was studied by alkaline hydrolysis and lactic acid-glycerine-acid magenta staining method.Results:In Hunyuan county,twelve strains of mycorrhizal mycospores belonging to three genera were obtained from rhizosphere soil of three years wild Astragalus membranaceus(Fisch.)Bge.var.mongholicus and the mean infection rate was 33.4%.On the campus of Shanxi university of Chinese medicine,six strains of mycorrhizal mycospores belonging to three genera were obtained from rhizosphere soil of three years growing Astragalus membranaceus and the mean infection rate was 9.6%.Conclusion:The species of mycorrhizal fungi and the average infection rate were different in different habitats.