一种新型荧光探针的设计、合成及其传感性能

Design, Synthesis and Properties of A Novel Fluorescent Probe

以吖啶酮为荧光团,通过与特定的金属离子配位实现抑制C=N异构化达到关-开识别的目的,设计并合成了一种新型荧光探针,4-[(吡咯-2-亚甲基)氨基]吖啶-9(10 H)-酮(4-AAP),结构经过氢谱和碳谱表征。性能研究表明,在V(N,N-二甲基甲酰胺)∶V(甲醇)∶V(水)=1∶7∶2的混合溶液中,4-AAP能够选择性识别Zn2+,同时具有较强的抗干扰能力,荧光滴定实验和Job’s plot实验表明4-AAP与Zn2+的配位关系为2∶1(物质的量比),最低检出限为4.98×10^-7 mol/L。

Based on acridone fluorophore,a novel fluorescent probe,4-[(pyrrole-2-ylmethylene)amino]-acridin-9(10 H)-one(4-AAP),was designed and synthesized in order to achieve“off-on”goal by inhibiting C=N isomerization coordinated with sepecial metal ion.The structure of 4-AAP was characterized by 1 H NMR and 13 C NMR.The properties showed that 4-AAP could selectivity recognize Zn2+in the mixture of V(DMSO)∶V(methanol)∶V(H2O)=1∶7∶2.Meanwhile,4-AAP has a strong anti-interference ability.The experiment of fluorescence titration and Job’s plot indicated that the coordination ratio of 4-AAP and Zn2+was 2∶1(The ratio of moles)and the detection limit was 4.98×10^-7 mol/L.