几种盐生植物与甜生植物NHX1生物信息学分析

Bioinformatics Analysis of Several Halophytes and Glycophyles NHX1

为了研究盐生植物与甜土植物Na^+/H^+逆向转运蛋白1 (NHX1)在结构及功能方面的差异,本研究采用生物信息学方法预测和分析甜土植物(拟南芥,水稻,小麦)与盐生植物(角果碱蓬,滨藜,盐角草)的Na^+/H^+逆向转运蛋白1 (NHX1)的基本性质、亲疏水性、N-糖基化位点、磷酸化位点、跨膜结构及其同源性关系。结果显示,盐生植物与甜土植物NHX1基因基本理化性质基本相同,都是稳定蛋白,氨基酸组成、亲疏水性差异不明显。但是N-糖基化位点、磷酸化位点、跨膜结构、多重序列比对分析存在明显的差异。上述研究结果可以为后续耐盐碱作物的分子植物育种提供一定的理论基础。

In order to study the difference in structure and function between Na^+/H^+antiporter 1(NHX1) in halophyte and glycophyte, this paper uses bioinformatics method to predict and analyze glycophytes(Arabidopsis, rice,wheat) and salt. The basic properties, hydrophilicity, N-glycosylation site, phosphorylation site, transmembrane structure and homology of NHX1 in plants(Suaeda corniculata, Atriplex patens, Salicornia europaea). The results showed that the basic physical and chemical properties of the NHX1 gene of the halophyte and glycophytes were basically the same, all of which were stable proteins, and the difference in amino acid composition and hydrophilicity was not obvious. However, there are significant differences in N-glycosylation sites, phosphorylation sites, transmembrane structures, and multiple sequence alignment analysis. The above research results can provide a theoretical basis for molecular plant breeding of subsequent salt-tolerant crops.