基于表型性状初步构建格木核心种质

Preliminary Construction of Core Collection of Erythrophleum fordii Based on Phenotypic Traits

构建格木核心种质,去除收集材料中的冗余样品,为格木种质资源的保存、研究和利用提供依据。本研究将114份格木种质按地理区域分组,通过测量果荚和种子的13个表型性状,在系统聚类和优先取样法的基础上,利用遗传多样性法、比例法和对数法3种方法,设定10%、20%和30%3种取样比例,共产生7种取样策略。采用极差符合率(CR)、最小值变化率(CRMIN)、最大值变化率(CRMAX)、变异系数变化率(VR)、均值差异百分率(MD)、方差差异百分率(VD)、平均值变化率(CRMEA)和平均表型多样性指数(MI) 8个参数评价7种策略构建的核心种质,以选出参数最优的核心种质。通过比较核心种质与原始种质的表型多样性指数、符合率、主成分和样品分布图,验证所构建核心种质的代表性。结果表明:(1)采用比例法在20%的取样比例时形成的核心种质参数最优。(2)在8个参数分别为100.00%、100.00%、100.00%、138.85%、0.00%、61.54%、99.29%和2.68时,核心种质的最终取样比例为21.05%。(3)核心种质与原始种质在13个表型性状上的多样性指数经t检验,差异均不显著;均值符合率在97.39%~99.98%之间,极大值、极小值符合率为100%,多样性指数符合率在90.34%~99.56%之间。原始种质和核心种质4个主成分的累积贡献率分别为87.382%和88.206%;两者的样品分布图具有相似的分布结构。以上表明获得的24份核心材料较好地代表了原始种质的表型性状变异特征。

Corecollection of Erythrophleum fordii was constructed to retrench germplasms had collected, which will provide a theoretical basis for preservation, utilizing and studying germplasm of Erythrophleum fordii. 114 germplasms of E. fordii grouping by geographical area were used as test materials. Through the measurement of 13 related phenotypic traits of pods and seeds of E. fordii, based on system cluster and preferred method, applied genetic diversity, proportional and logarithmic method, 10%, 20% and 30% sample ratio, total seven sampling strategies were formed. The sampling strategies were tested by the value of CR, CRMIN, CRMAX, VR, MD, VD,CRMEA, MI. By comparing the phenotypic diversity index, coincidence rate and principal component and distribution map of the core collection and total collection, the core collection was tested by representativeness. The results wereas follows:(1)The optimal strategy is: proportional method and 20% sampling ratio.(2) The values above are 100.00%, 100.00%, 100.00%, 138.85%, 0.00%, 61.54%, 99.29% and 2.68, respectively. The final sampling ratio of core collection is 21.05%.(3) The diversity index of 13 phenotypic traits of core collection and total collection is not significant by t test. The average coincidence rate between the core collection and total collection on the 13 indexes is between 97.39% ~99.98%, the maximum and mini mum coincidence rate is 100%, and the diversity index coincidence rate is between 90.34%~99.56%. There are 4 principal components of core collection and total collection, the accumulative contribution rates are 87.382% and 88.206%, respectively. The sampling distribution map of core collection and total collection is similar. The obtained core collection of 24 E. fordii could satisfactorily represent the phenotypic variation characteristics of 114 E. fordii germplasms.