基于ISSR分子标记的早熟禾种质资源遗传多样性分析

Genetic Diversity Analysis of Bluegrass Germplasm Based on ISSR Markers

本研究以青海和西藏不同地理位置的60份早熟禾种质资源为材料,通过ISSR分子标记进行遗传多样性分析,以期了解青藏高原主体地区早熟禾种质资源间遗传关系,为种质创新及新品种选育提供理论依据。结果表明,在30条ISSR引物中共扩增出297条谱带,其中多态性谱带296条,多态性比率达到99.66%;等位基因平均值(na*)为1.996 6,有效等位基因平均值(ne*)为1.539 3,Nei’s基因指数平均值(h*)为0.320 1,shannon’s信息指数平均值(I*)为0.484 7;遗传相似系数阈值范围在0.525 3~0.878 8之间;根据UPGMA聚类分析,在遗传相似系数0.61处,60份早熟禾种质材料被聚为4大类,其中A大类、B大类和C大类分别占总量的76.7%、5%、16.7%,D大类只有一份材料单独聚为一类;整个聚类分析中,大部分来自相近或者相同地理区域的种质材料聚于同一类或者亚类,但种质材料间也有交叉现象。

This study was based on 60 of the Poa L. germplasm resources from the Tibetan Platea, using the method of ISSR molecular markers for genetic diversity analysis. In order to understand the genetic differences among Poa L. germplasm. It provides a scientific basis for the rational utilization of the Poa L. germplasm resources and the breeding of new varieties for the Tibetan Platea. Total 297 bands were amplified by use of 30 ISSR primers, in which 296 were polymorphic bands and the polymorphism rate was 99.66%. The average number of tested alleles(na*) was 1.996 6. The effective alleles(ne*) was 1.539 3. Nei’s gene diversity index(h*) was0.320 1, and Shannon’s information index was 0.484 7. The genetic similarity was 0.525 3~0.878 8. Using UPGMA cluster analysis, the genetic similarity coefficient of 0.61 was used as the boundary, and the accessions were divided into 4 types, among them, category A and category C accounted for 76.7% and 16.7% respectively, the D category consists of only one single material grouped into one category. The whole cluster analysis, most of the germplasm materials from similar or similar geographical areas are clustered in the same class or subclass, but there is also a cross between germplasm. It is indicated that the Poa L. germplasm material is rich in genetic diversity, and provides the theoretical basis for the identification and classification of the Poa L. germplasm resources from molecular level.